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    HUMAN DOSES OF DEHP DURING PREGNANCY INFLUENCE POST-NATAL GROWTH AND REPRODUCTION IN OFF-SPRING FEMALE MICE
    (Hajee Mohammad Danesh Science & Technology University, Dinajpur-5200, Bangladesh, 2023-12) HASAN-UZ-ZAMAN
    Di (2-ethylhexyl) phthalate (DEHP) is an endocrine-disrupting chemical that interferes with reproductive function and hormone regulation. Among different exposure routes (oral ingestion, inhalation, and dermal contact), the most common routes of exposure are via oral ingestion from food packaging and the use of cosmetic products, but high levels of phthalates are also present in household dust. Thus, exposure to phthalates is ubiquitous in human populations. For the general population, it was estimated that the average total daily individual ambient exposure to DEHP ranges from 0.21 to 2.1 mg/day. Thus, the estimated range of daily human exposure to DEHP is 3–30 μg/kg/day based on urinary metabolite concentrations; however, measurements of DEHP in household dust can reach up to 700 mg/kg, potentially increasing exposure levels in certain individuals. The effect of phthalate on male reproduction is now well studied; however, the effect of phthalate on female reproduction warrants extensive investigation. Because women are exposed to phthalates at a higher level than men through their widespread use of personal care and cosmetic products, In addition, it is hypothesized that phthalate can cross the placenta and interfere with the embryo, postnatal growth and development, and the later life of offspring after parturition. In this study, the Swiss albino female mice were randomly divided into four groups namely T0 (control), T1 (2mg/kg DEHP), T2 (4mg/kg DEHP), and T3 (6 mg/kg DEHP). Mating‟s strategy was followed by two virgin females and one stud male, who were confirmed pregnant by observing a vaginal plug. DEHP was exposed from the plug confirmed to parturition. After parturition, litter size (number of pups), litter weight, weight/pups, and body weight took until sexual maturity, then evaluated the reproductive cyclicity, the hormonal profile, hematological parameters, and organ developments after sexual maturity of female mice. The result showed that from birth to weaning, offspring of the T1 group exhibited a significantly body weight gain compare to the T0 group. On the other hand, considering weaning to sexual maturity, the rate of body weight gain was higher in the T1 and T2 groups compared to the T0 group. The final body weight was higher in the T2 group compared to the offspring of the T0 group. The estrous cyclicity data revealed that the rate of estrous in the T1, T2, and T3 groups were gradually decreased compared to the T0 group, in case of proestrus T1 was increased compared to T0 group but T2, T3 group were decreased compared to T0 group. The rate of diestrus in the T1, T2 and T3 groups were increased compare to the T0 group. On the other hand the rate of metestrus in the T1 and T2 group were decreased compare to T0 group but T3 was increased compared to T0 group. In the hormonal assay, the percentage of estrogen in the T1, T2 and T3 were gradually decreased compared to T0 group and the percentages of progesterone levels in the treatment groups were decreased significantly. Hematological parameters showed that the platelets in the T1, T2, and T3 groups were significantly higher than T0 group. In the term of neutrophil percentage, the T3 group was significantly lower than the other groups, Lymphocyte percentage in the T1, T2, and T3 groups were significantly higher than the T0 group. In case of total WBC count in the T1, T2 and T3 groups were increased compared to T0 group. The hematological parameters of PCT, RDW-CV, and RDW-SD were significantly lower in the T1, T2, and T3 groups than the T0 group. The percentage of monocytes in the T1, T2 and T3 were gradually decreased compared to T0 group. In cases of organ development, lung weight in the T1 and T3 groups were significantly increased from the T0 group but the T2 group was decreased from T0 group. In the T1, T2 and T3 groups, kidney and liver weight were significantly higher than in the T0 group. Heart weight in the T2 and T3 groups were significantly higher than in the T0 group. In the same way, spleen weight is comparatively higher in the T1 and T2 groups compared to the T0 group. There were no significant changes in the thyroid, uterus, or ovaries. Visual observation of the morphological characteristics of different organs showed that slight discoloration of the liver and lung was substantially affected in T3 group compared to T0 group.
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    EFFECT OF NITROGEN AND PHOSPHORUS ON THE GROWTH AND YIELD OF TOMATO
    (Hajee Mohammad Danesh Science & Technology University, Dinajpur-5200, Bangladesh, 2025-06) BAPPY, MD. RAKEBUL ISLAM
    A pot experiment was conducted inside the net house at Hajee Mohammad Danesh Science and Technology University (HSTU), Dinajpur during the period of November 2024 to February 2025, to study the effect of nitrogen and phosphorus on growth and yield of tomato. The experiment was laid out in a Complete Randomized Design (CRD) having seven treatments with three replications: T1: N0P0, T2: N1P1, T3: N1P2, T4: N2P1, T5: N2P2, T6: N3P1, T7: N3P2 .Different doses of N (0, 0.35, 0.45, 0.55 g pot-1) and P(0, 0.20, 0.25 g pot-1) were applied in soil based on 0, 80, 100, 120 kg ha-1 and 0, 45, 50 kg ha-1 respectively in soil. Nitrogen and phosphorus fertilization to the soil influenced the plant growth and yield contributing characters of tomato. The highest value of different parameters such as plant height (The maximum plant height 66.33, 91.67, 106.00 and 118.00 cm was observed in T7 treatment at 30 , 45 ,60 DAT and at harvest respectively), number of leaf plant-1 (14.33, 17.00, 19.33 was observed in T7 at 30, 45 and 60 DAT respectively), number of branches (5.99 and 6.67 was observed in T7 treatment at 45 and 60 DAT respectively), number of flowers plant-1 ( 33.00, 46.00 was observed in T7 at 45 and 60 DAT respectively), no of fruit plant-1 (43.33 was observed in T7 ), weight of fruit (2.10 kg plant-1 was observed in T7 treatment), root length (24.40 cm was observed in T7 ), fresh root weight (14.27 g plant-1 was observed in T7), dry weight of root (4.33 g plant-1 was observed in T7), fresh and dry weight of shoot (305.73 g plant 1 and 16.36 g plant-1) both were observed in T7 treatment. The lowest values of all the parameter of growth and yield contributing characters were found in control T1 treatment. Although highest amount of nutrient in the fruit like Ca, Mg and K (133.00, 88.00 and 106.87 mg/1000gm) fruit were observed in T2 treatment and lowest amount of nutrient in the fruit were observed in T7 treatment. It is showed that the performance of the treatment T7 was the best among all the other treatment. Therefore, the study reveals that the soil application of 120 kg N ha-1 and 50 kg P ha-1 might be an efficient practice for achieving economical tomato production but increasing amount N and P application reduced chemical content (Ca, Mg, K) of tomato fruit.
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    EFFECT OF SILICON (SiO2) TO CONTROL BIPOLARIS LEAF BLIGHT AND TO IMPROVE SEED HEALTH STATUS OF WHEAT
    (Hajee Mohammad Danesh Science & Technology University, Dinajpur-5200, Bangladesh, 2023-12) ROY, APURBA
    The experiment was conducted in the research field of Hajee Mohammad Danesh Science and Technology University, Dinajpur from December 2022 to April 2023 to evaluate the effectiveness of various doses of silicon dioxide (SiO2) as treatment to control leaf blight (Bipolaris sorokiniana) and to improve seed health status of wheat. In this experiment, three treatments were studied, T1 = Silicon dioxide @60 kg ha-1, T2 = Silicon dioxide @120 kg ha-1, T3= Silicon dioxide @180 kg ha-1. Two varieties were used, V1= BWMRI Gom-1 and V2= BWMRI Gom-2 within three replications and the experiment was set up as Randomized Complete Block Design (RCBD). Bipolaris leaf blight severity, seed health (seed number associated with pathogens and percent germination), plant height, spike length, thousands seed weight, biological yield, yield index and yield were significantly varied with different treatments. Minimum disease severity was recorded from Silicon dioxide @60 kg ha-1 on flag leaves at 67 DAS (2.3%), 72 DAS (6.44%), 77 DAS (6.77%), 82 DAS (8.35%) also on flag-1 leaves at 67 DAS (1.54%), 72 DAS (3.31%), 77 DAS (5.97%) and 82 DAS (11.12%). Minimum disease severity was recorded in BWMRI Gom-1 on flag leaf at 67 DAS (1.90%), 72 DAS (4.55%), 77 DAS (5.69%), 82 DAS (9.01%) and also on flag-1 leaves at 67 DAS (0.67%), 72 DAS (2.15%), 77 DAS (5.68%) and 82 DAS (10.19%). As a treatment variety interaction, the lowest disease severity on flag leaf was recorded from BWMRI Gom-1 with silicon dioxide @60 kg ha-1 interaction at 67 DAS (1.39%), 77 DAS (5.12%), 82 DAS (7.03%) and from BWMRI Gom-1 with Silicon dioxide @120 kg ha-1 interaction at 72 DAS (3.80%). Also the lowest disease severity on flag-1 leaves was recorded from BWMRI Gom-1 with Silicon dioxide @60 kg ha-1 at 67 DAS (0.0%), 72 DAS (1.11%), 77 DAS (3.72%) and 82 DAS (5.92%). As a treatment, minimum seed number was affected with Bipolaris sorokiniana was recorded from Silicon dioxide @60 kg ha-1 (10.13%). As a variety, minimum seed number was affected with Bipolaris sorokiniana was recorded from BWMRI Gom-1 (10.92%). Treatment variety interaction of BWMRI Gom-1 with Silicon dioxide @60 kg ha-1 showed minimum (8.18%) seed number was affected with Bipolaris sorokiniana. Highest grain yield (4.72 t ha-1) was obtained from silicon dioxide @60 kg ha-1. Maximum grain yield (4.36 t ha 1 ) was produced from BWMRI Gom-1. The highest grain yield (4.76 t ha-1) was obtained from the interaction of BWMRI Gom-1 with Silicon dioxide @60 kg ha-1. Finally, the research study concluded that using of BWMRI Gom-1 with the application of Silicon dioxide @60 kg ha-1 can be used effectively with lowest leaf blight disease infestation with better yield.
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    EFFECTS OF ORGANIC AMENDMENTS ASSISTED PHYTOREMEDIATION POTENTIAL OF LEAD CONTAMINATED SOIL BY MAIZE
    (Hajee Mohammad Danesh Science & Technology University, Dinajpur-5200, Bangladesh, 2023-12) TONNI, MST. NURE FATEMA
    Phytoremediation, the use of plants and their associated microbes for environmental cleanup, has gained acceptance in the past 10 years as a cost-effective, noninvasive alternative or complementary technology for engineering-based remediation methods. Plants can be used for pollutant stabilization, extraction, degradation, or volatilization. This study evaluated the effects of organic amendments to enhance phytoextraction of heavy metal lead (Pb) from artificially polluted soil by maize (Zea mays). The objective of this study was to investigate the impacts of altered soil chemical properties (soil pH, OM, cation exchange capacity, electrical conductivity, base cation (Ca, Mg, K and Na) on phytoremediation of Pb contaminated soil due to application of compost, biochar, ash, poultry litter and co-compost. The treatments were a) control with 200mg Pb, b) Pb contaminated soil with compost 100g (10%), c) Pb contaminated soil with ash 100g(10%), d) Pb contaminated soil with biochar 100g (10%), e) Pb contaminated soil with poultry litter 100g (10%), f) Pb contaminated soil with co-compost (10%) and replicate three times in a completely randomized design. The results indicated thatthe compost, poultry litter significantly enhanced the growth ratio and plant heightof maize under Pb contaminated environment. Ash and biochar increased plant height same as control. Biochar and COMBI increased total amount of plant available P and N. Compost, ash, poultry litter showed the same effect on total phosphorus and nitrogen which was greater than the control. In case of soil pH, the sequence is: poultry litter> COMBI> compost> biochar>ash>control. For soil OM: compost>COMBI> biochar> control> poultry litter>ash. For soil EC: compost>poultry litter>COMBI>ash> biochar>control and soil CEC: compost>poultry litter>COMBI>biochar>ash>control. Moreover, compost enhanced base cations like Ca, Mg greater than ash, biochar, poultry litter, co-compost. Poultry litter accelerated base cations like K, Na compared to control. Therefore, it can be suggested that application of organic amendments like biochar, compost, ash, poultry litter, co-compost was the most suitable choice for the treatment of Pb contaminated soil phytoremediation.
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    EFFECT OF DIFFERENT ORGANIC FERTILIZERS ON NAPA SHAK (Malva verticillata L.)
    (Hajee Mohammad Danesh Science & Technology University, Dinajpur-5200, Bangladesh, 2023-12) RINA, MOST. AKTER
    An experimental study was carried out in the Soil Science Research Field of Hajee Mohammad Danesh Science and Technology University (HSTU) in Dinajpur, Bangladesh, from November 2022 to February 2023. The objective was to assess the effects of several organic manures, as well as their combination, on the growth of Napa Shak. The investigation encompassed six distinct treatments: T1 = Control, T2 = Compost (2.5 t ha-1), T3 = Cow dung (5 tha-1), T4 = Vermicompost (1.25 t ha-1), T5 = Northan organic fertilizer (0.50 t ha-1), T6 = Poultry manure (5 t ha-1) was applied. The experiment follows a randomized complete block design (RCBD). Data was gathered on the following parameters: plant height (in cm), fresh shoot weight (in g), fresh root weight (in g), and the presence of post-harvest soil S (in ppm), P (in ppm), exchangeable K (in meq 100 g-1 of soil), total N (%), pH, OC (%), and OM (%). The study analyzed the impact of different organic fertilizers on the growth of Napa Shak. The highest plant height was observed at 15 DAS, 30 DAS and harvest, with T6 (poultry manure) having the highest height at 14.13, 17.40 and 21.43 cm respectively and lowest from T1was 6.37, 8.40 and 8.40 cm respectively. The highest fresh weight (3.28 g) was also observed, with T6 having the highest root weight (0.16 g). The highest N content was found in T6 at 0.16 %, followed by T5, T4, and T3 and the lowest from T1 0.09%. The highest available P was 24.33 ppm from T6 and the lowest from T1 (9.68 ppm) with having the highest available S at 16.08 ppm and lowest from T1 (7.03 ppm). T6 also had the highest OC and OM values at 0.67 and 1.17 %, with T6 treatment following T5, T3, T4, and T2 were 0.58, 0.56, 0.54 and 0.54 % respectively in terms of OC and OM and lowest form T1 (control). The study concluded that T6 fertilizer poultry manure at a rate of 5 t ha -1 produced the maximum growth of Napa Shak. Further research is needed to determine the regional adaptability of this study.
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    UTILIZATION OF AVAILABLE ONLINE-BASED ORGANIC FERTILIZERS ON THE VEGETATIVE GROWTH OF WATER SPINACH
    (Hajee Mohammad Danesh Science & Technology University, Dinajpur-5200, Bangladesh, 2023-12) ANNY, MAHBUBA ALAM
    The experiment was conducted at the shade besides Academic building-1 of Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, from November 2022 to March 2023 to study the utilization of available online-based organic fertilizers on the vegetative growth of water spinach. The experiment was laid out Completely Randomized Design (CRD) with 8 treatments and 3 replications. A total of 24 experimental pots were used. Every treatment used as 1% Kg-1 soil. There were eight treatments as follows: T1 = Control (only soil), T2 = 1% Bonemeal Kg-1 soil, T3 = 1% Cowdung Kg-1 soil, T4 = 1% Neem cake Kg-1 soil, T5 = 1% Oyster shell powder Kg-1 soil, T6 = 1% Cocopeat Kg-1 soil, T7 = 1% Vermicompost Kg-1 soil, T8 = 1% Egg shell powder Kg-1 soil. The results showed that the vegetative growth and yield attributing components such as plant height, number of leaves plant-1, fresh shoot biomass, dry shoot biomass, fresh root biomass, dry root biomass responded significantly except plant and no of leaves at early stages due to the application of available online based organic fertilizers. The highest values of different parameters such as plant height (12.40 cm) in T5 treatment at 50 DAS considered as best time of economic yield, fresh shoot biomass (30.95 g) and dry shoot biomass (4.64 g) in T6 treatment due to 1% cocopeat Kg-1 soil application, fresh root biomass (2.27 g) and dry root biomass (0.34 g) was obtained in T3 treatment where 1% Cowdung Kg-1 soil were applied. The lowest results were found in the control treatment (T1). A significant increase in the N contents in leaves, shoots and roots as well as their uptake were observed due to the different treatments. Treatment T6 performed the highest yield as a result of 1% cocopeat Kg-1 soil application. Treatment T5 composed of 1% Oyster shell Kg-1 soil gave the highest post-harvest soil pH and available S. The highest total N, available P, exchangeable K and OC noticed in T7 (0.04), T2 (25.30), T6 (0.19) and T4 (0.97), respectively might be better for sustainable soil health for growing crops. Overall result suggested that incorporation of 1% cocopeat Kg-1 soil can be better for cultivation of Water spinach in respect to higher yield response and might be efficient cultivation.
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    RESIDUAL EFFECT OF BIOCHAR WITH REDUCED DOSES OF CHEMICAL FERTILIZERS ON THE SOIL PROPERTIES, GROWTH AND YIELD RESPONSE OF OKRA
    (Hajee Mohammad Danesh Science & Technology University, Dinajpur-5200, Bangladesh, 2023-12) JANNAT, MOST. TANBIN
    The research work was conducted in the research field of the Department of Soil Science, Hajee Mohammad Danesh Science and Technology University (HSTU), Dinajpur during November 2022 to January 2023 to study the residual effect of biochar with reduced doses of chemical fertilizers on the soil properties, growth and yield response of okra (Local variety: Heera). The experimental soil belongs to the Old Himalayan Piedmont Plain (AEZ-1). The experiment was laid out in the randomized complete block design (RCBD) having eleven (11) treatments with three replications. The treatments were T1 = No residual biochar + No chemical fertilizer (Control), T2 = 100% RDF (Recommended Dose of Fertilizers), T3 = 50% RDF + Residual 20 t ha-1 Rice Husk biochar, T4 = 50% RDF + Residual 20 t ha-1 Sawdust biochar, T5 = 50% RDF + Residual 20 t ha-1 Biochar enriched organic fertilizer (OF), T6 = 75% RDF + Residual 15 t ha-1 Rice Husk biochar, T7 = 75 % RDF + Residual 15 t ha-1 Sawdust biochar, T8 = 75% RDF + Residual 15 t ha-1 Biochar enriched organic fertilizer (OF), T9 = 100% RDF + Residual 10 t ha-1 Rice Husk biochar, T10 = 100% RDF + Residual 10 t ha-1 Sawdust biochar, T11 = 100% RDF + Residual 10 t ha-1 Biochar enriched organic fertilizer (OF). The results showed that the growth and yield attributing components such as plant height, number of fruit plant-1, marketable fruit weight plant-1, fruit weight plot-1, fruit length, fresh shoot biomass plant-1, dry shoot biomass plant-1, root length, fresh root weight, dry root weight responded significantly except fruit breadth due to the application of different level of chemical fertilizers along with different level of residual biochar combination. The highest values of different parameters such as plant height (74.2 cm), number of fruit plant-1 (16.6), marketable fruit weight plant-1 (197.8 g), fruit weight plot-1 (1.18 kg), fruit length (11.3 cm), fruit breadth (5.0 cm), fresh shoot biomass plant-1 (181.5 g), dry shoot biomass plant-1 (30.8 g) were obtained in T11 treatment where 100% RDF + Residual 10 t ha-1 Biochar enriched organic fertilizer (OF) were applied. The lowest results were found in the control treatment (T1). In post- harvest soil, the treatments (T4 and T5) receiving high amount (20 t ha-1) of biochar gave the highest amount of total N, available P, exchangeable K, available S, organic carbon (OC) and organic matter (OM). In case of pH the highest (7.31) was found in T5 treatment. The residual effect of biochar with reduced doses of chemical fertilizers might be better for sustainable soil health for growing crops. Overall, the results suggested that reduced doses of chemical fertilizers in previous biochar incorporated field can be better for cultivation of okra in respect to higher yield response and might be advantageous cultivation of crops in the next year.
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    EFFECTS OF BORON ON THE GROWTH AND YIELD OF SOYBEAN
    (Hajee Mohammad Danesh Science & Technology University, Dinajpur-5200, Bangladesh, 2023-12) JAYHAN, UMMAY
    A field experiment was conducted at the Soil Science Research Field, Hajee Mohammed Danesh Science and Technology University (HSTU), Dinajpur during the period from March 2022 to May 2022 to investigate the effect of B on the growth and yield of soybean. The variety BARI Soyabean-5 was used as the test crop. The experiment comprised of 7 treatments: T1 = Control, T2 = 2 kg B ha-1, T3 = 4 kg B ha-1, T4 = 6 kg B ha-1, T5 = 8 kg B ha-1, T6 = 10 kg B ha-1, T7 = 12 kg B ha-1. The experiment was laid out in Randomized Complete Block Design (RCBD) with three replications. Different levels of B fertilization influenced the plant growth characters. Both the growth and yield increased with increasing the B level at certain level. In case of growth parameters, T5 (8 kg B ha-1) showed the maximum plant height (51.8, 65.7 and 82.0 cm at 30, 60 and 90 DAS, respectively), highest number of branches (4 at 90 DAS), number of leaves (13.11, 24 and 32.44 at 30, 60 and 90 DAS, respectively). Maximum pod number plant-1, maximum grain weight (1.64-t ha-1) and the highest average value for stover fresh and dry weight (8.60 and 6.75 t ha-1, respectively). Furthermost the highest pH value (6.60), total N (0.035%), available P, exchangeable K and available S (50.51 ppm, 16.91 meq 100g-1 soil and 8.22 ppm, respectively). The highest number of branches (2.22) at 60 DAS was found for T6 which was statistically similar with T5 and T7; the highest available B (0.34 ppm) for T7 (12 kg B ha-1) which was statistically similar with T5 and T6 treatment. Conversely, the control treatment (T1), without B supplementation, exhibited the minimum growth and development as well as the minimum pod number plant-1 (46.50) and the lowest grain weight (0.68 t ha-1) and also the least average value for stover fresh and dry weight (3.00 and 2.19 t ha-1, respectively) and lowest post-harvest soil nutrient that means a slight increase than the initial status. Therefore, the present experimental results suggest that the application of 8 kg B ha-1 with RDF would be beneficial to increase the seed yield of soybean variety BARI Soyabean-5 under the climatic and edaphic condition of HSTU, Dinajpur (AEZ-1).
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    YIELD STABILITY ANALYSIS BY AMMI MODEL AND ESTIMATION GENOTYPE × ENVIRONMENT INTERACTION OF BORO RICE (Oryza sativa) IN BANGLADESH
    (Hajee Mohammad Danesh Science & Technology University, Dinajpur-5200, Bangladesh, 2023-12) RANA, MD. MASUD
    Considering the yield stability of potential rice genotypes is the essential stage for perfect evaluation. Therefore, this study determined the Genotype × Environment Interaction (GEI) and yield stability performance of 10 boro rice genotypes in four environments namely Agronomy Research Field of Hajee Mohammad Danesh Science and Technology University, Dinajpur; Bangladesh Rice Research Institute (BRRI) Rangpur; Agronomy Research Field, Bangladesh Agricultural University, Mymensingh and Agronomy Research Field of Sylhet Agricultural University through 2022 boro season. The experiment used Completely Randomized Design (CRD) with three replications. Combined analysis and Additive Main effects and Multiplicative Interaction (AMMI) model analyzed yield stability performance. ANOVA result showed that there were highly significant differences among the genotypes for maximum characters. The AMMI analysis showed the environment explained 10.23% whereas the GEI accounted for 9.17% and the genotypes captured 80.60 % which significantly affected the grain yield. The environment, genotype main effects, and the GEI were all highly significant. The study indicated that the tested genotypes, BRRI dhan 89 (6922 kg ha-1) with a high and BRRI dhan 68 gave the lowest yield (5618 kg ha-1), respectively. Also genotypes BRRI dhan 100, BRRI dhan 28, and BRRI dhan 67 gave the highest grain yield and Dinajpur environment with a high mean value of (7556 kg ha-1) had great stability across the four environments. Therefore, the AMMI model might be a valuable tool for identifying the most suited and stable high yielding boro rice genotypes for wide regions in Bangladesh as well as for varied environmental conditions.
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    IMPACT OF ZINC OXIDE (ZnO) NANOPARTICLES ON THE GROWTH, PHYSIOLOGY, YIELD AND BIOCHEMICAL TRAITS OF WHEAT (Triticum aestivum L.) UNDER SALT STRESS
    (Hajee Mohammad Danesh Science & Technology University, Dinajpur-5200, Bangladesh, 2023-12) Hossain, Md. Motakabbir
    Worldwide, the productivity of various cereal crops is adversely affected by potential threat, with salinity stress being a significant factor. To alleviate the negative effects of salt stress on plants, a promising strategy is the application of ZnO nanoparticles through spraying. Although their effectiveness has been noted, the specific mechanism by which nano-ZnO particles operate to counter salt stress is not yet fully understood. Concerning this issue, a pot experiment was carried out at the Net house, Department of Agronomy, Hajee Mohammad Danesh Science and Technology University (HSTU), Dinajpur, Bangladesh during November 2022 to March 2023. The experiment encompassed three tiers of salt stress (0, 100, and 150 mM NaCl stress) and three levels of ZnO (0, 150, and 300 µM), resulting in nine distinct treatments, namely: i) S0ZnO0 (0 mM NaCl + 0 µM ZnO), ii) S0ZnO1(0 mM NaCl + 150 µM ZnO), iii) S0ZnO2 (0 mM NaCl +300 µM ZnO), iv) S1ZnO0 (100 mM NaCl + 0 µM ZnO), v) S1ZnO1 (100 mM NaCl + 150 µM ZnO), vi) S1ZnO2 (100 mM NaCl + 300 µM ZnO), vii) S2ZnO0 (150 mM NaCl + 0 µM ZnO), viii) S2ZnO1 (150 mM NaCl + 150 µM ZnO), ix) S2ZnO2 (150 mM NaCl + 300 µM ZnO). The main findings showed that the studied parameters such plant morphology (plant height, number of leaves, leaf area), growth (fresh & dry weight of leaves, stems and roots), phenology (days to booting, heading, anthesis and physiological maturity), physiology (photosynthetic pigments; water status-RWC, WSD, WRC, WUC; MSI), yield contributing traits (spike length, number of spikelets spike-1, grains spike-1, and hundred grains weight), yield indices (grain, straw and biological yield, and harvest index) and biochemical traits were negatively and progressively affected with increasing salt stress. Following the application of nano-ZnO particles, enhancements were observed in the aforementioned plant traits. Notably, ZnO2 (300 µM ZnO) demonstrated the most favorable results, although its performance was nearly statistically indistinguishable from ZnO1 (150 µM ZnO). Furthermore, the results showed that although SS raised the Na content while lowering the K, Ca, N, and protein content, ZnO administration increased the K, Ca, N, and protein values while falling the Na values. The application of nano-Zn particles has demonstrated the potential to enhance plant development by alleviating salt stress, with 150 µM ZnO identified as an effective dosage.