DETERMINATION OF AN IDEAL EXPLANT, HORMONAL COMBINATIONS FOR IN VITRO REGENERATION OF SOYBEAN (Glycine max (L.) Merr.)

Abstract

The experiment was carried out during the period from December 2009 to April 2010, in the tissue culture laboratory of the Department of Genetics and Plant Breeding, Hajee Mohammad Danesh Science and Technology University, Dinajpur to determine an ideal explant and hormonal combination for in vitro regeneration of soybean. The effects of explant (hypocotyl and leaf) and different concentrations of Kinetin (0.0, 1.0, 1.5 and 2.0 mg/l) and NAA (0.0, 1.0, 1.5 and 2.0 mg/l) on callus induction was investigated. Between the two explants, hypocotyl explants were superior to the leaves considering all the parameters for callus induction. The best result obtained from MS medium supplemented with 1.0 mg/l] Kinetin and 2.0 mg/l NAA. Tissue culture derived callus from hypocotyl and leaf were transferred to MS medium supplemented with different concentrations of BAP (0.0, 1.0, 2.0, 3.0 and 4.0 mg/l) with 0.5 mg/l NAA for shoot induction. MS medium supplemented with 3.0 mg/l BAP and 0.5 mg/l NAA showed best result for shoot induction. Incase of shoot development leaf derived calli performed better than those derived from hypocotyls. Shoot developed roots on MS medium supplemented with different concentrations of IBA (0.0, 1.0, 1.5, 2.0 and 3.0 mg/l) where 2.0 mg/l showed the best result for rooting. Shoot developed from leaf calli performed better considering all the parameters for root induction. Finally the rooted plants were hardened and transferred to pot.