DETERMINATION OF AN IDEAL EXPLANT, HORMONAL COMBINATIONS FOR IN VITRO REGENERATION OF SOYBEAN (Glycine max (L.) Merr.)
Date
2010-08
Authors
Journal Title
Journal ISSN
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Publisher
HAJEE MOHAMMOD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR
Abstract
The experiment was carried out during the period from December 2009 to April
2010, in the tissue culture laboratory of the Department of Genetics and Plant
Breeding, Hajee Mohammad Danesh Science and Technology University,
Dinajpur to determine an ideal explant and hormonal combination for in vitro
regeneration of soybean. The effects of explant (hypocotyl and leaf) and different
concentrations of Kinetin (0.0, 1.0, 1.5 and 2.0 mg/l) and NAA (0.0, 1.0, 1.5 and
2.0 mg/l) on callus induction was investigated. Between the two explants,
hypocotyl explants were superior to the leaves considering all the parameters for
callus induction. The best result obtained from MS medium supplemented with 1.0
mg/l] Kinetin and 2.0 mg/l NAA. Tissue culture derived callus from hypocotyl and
leaf were transferred to MS medium supplemented with different concentrations of
BAP (0.0, 1.0, 2.0, 3.0 and 4.0 mg/l) with 0.5 mg/l NAA for shoot induction. MS
medium supplemented with 3.0 mg/l BAP and 0.5 mg/l NAA showed best result
for shoot induction. Incase of shoot development leaf derived calli performed
better than those derived from hypocotyls. Shoot developed roots on MS medium
supplemented with different concentrations of IBA (0.0, 1.0, 1.5, 2.0 and 3.0 mg/l)
where 2.0 mg/l showed the best result for rooting. Shoot developed from leaf calli
performed better considering all the parameters for root induction. Finally the
rooted plants were hardened and transferred to pot.
Description
Soybean [Glycine max (L.) Merr.] is the most important legume crop all over the
world as it is the major source of edible oil and plant protein (Bonacin et al.,
2000). Soybean belongs to the family Leguminosae and sub-family Papilionaceae.
Soybean seeds contain 30.16% carbohydrate, 19.94% fat, 36.46% protein, 9.3%
dietary fiber and a good amount of calcium, magnesium, phosphorus, potassium,
zine and vitamins (vitamin A, Bs, By, C, K) and amino acids (tryptophan,
threonine, isoleucine, leucine, lysine, methionine, phenylalanine, tyrosine, valine,
arginine, histidine, alanine, aspertic acid, glutamic acid, glycine, proline, serine)
(USDA, 2007). The chemical composition of soybean seeds recommends it as one
of the main solutions for global food deficits (Bonacin et al., 2000). Soybean has
many kinds of anti-disease components, such as, isoflavone, protein, trypsin
inhibitor, phytic acid and saponin. Further more, soybean oil is low in saturated fat
and cholesterol (RDA, 2003).
Keywords
Plant materials, Culture media used, Preparation of culture media, Different steps of media preparation, Steps for the preparation of culture media