EVALUATION OF THE EFFECTIVENESS OF COMMERCIALLY AVAILABLE DISINFECTANTS AGAINST SALMONELLA ISOLATES FROM INTERNAL ORGANS OF DEAD BIRDS

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2010-08

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HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR.

Abstract

The present study was reflected on the isolation and identification of Salmonella from internal organs of dead birds belonging to the selected areas and evaluation of the effectiveness of commercially available disinfectants against Salmonella isolates. For isolation and identification of salmonella organisms from the internal organs (liver, spleen, heart and lungs) of dead birds were subjected to microbiological, cultural examination using microscopic studies and different biochemical tests. A total of 154 samples from dead birds were tested for the examination. Out of 154 samples 36 were found to be positive to Salmonella. Among the positive samples, 17 were from Basherhat, 12 were from Gobindapur and 7 were from Mohabolipur, Dinajpur. The percentages of positive samples from those areas were 24.28%, 22.22% and 23.33% respectively and the average prevalence of Salmonella in the study area was 23.37%. In case of organs specificity, Salmonella was detected from 18 samples of liver, 12 samples of spleen, 5 samples of heart and 1 sample of lung and the prevalence was 34.61%, 23.07%, 20.00 % & 4.0% respectively. In the result of my second experiment I found that Desinkap was most effective of all the disinfectants in preventing the growth Salmonella isolates by zone of inhibition after being incubated for 24 hours which was not statistically significant (P> 0.05). GPC (8) was found to be more effective than Virocid and TH4, but not more effective than Desinkap. Despite the fact that Desinkap was the most effective disinfectant because Desinkap contained a combination of the active ingredients formaldehyde, glutaraldehyde, glyoxal, benzylconium and it is likely that formaldehyde is the best active ingredient at preventing the growth of the Salmonella bacteria. Lysol disinfectant was less effective than other four disinfectants.

Description

The importance of Salmonellosis in poultry sector has increased to be growing concern day by day throughout the world during the last decades. The etiologic agents are responsible for various pathogenic processes in man and animals including poultry (Freeman, 1985). Since the direct and indirect losses due to this type of infectious agent are estimated undoubtedly very high, therefore the research for the epidemiology of infection, reservoir of salmonella infection and economic losses continue to be of increasing interest to the bacteriologists and epidemiologist all over the world.

Keywords

COMMERCIALLY AVAILABLE DISINFECTANTS, Sources of Salmonella infection, Media used for biochemical test

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