In vivo Investigation of CNS Depressant and Anxiolytic Activity of Fruits of Aphanamixis polystachya (Meliaceae)

Abstract

Plants and other natural substances have been used as the rich source of medicine. The list of drugs obtained from plant source is fairly extensive. Aphanamixis polystachya plant is reported in ethnopharmacological as medicinal plant. The neuropharmacological activity was evaluated by Hole cross, Hole board and Elevated plus-maze test and data were analyzed by using SPSS analytical method. For hole cross experiment n-hexane and methanol soluble fraction at dose of 200 and 400 mg/kg body weight, the decreased the locomotor activity of the experimented animals. Among these sample, n-hexane fraction of A. polystachya fruits at dose of 200 mg/kg body weight mostly decrease the frequency of moment of the mice through the hole. It was also observed that methanol fraction at dose of 400 mg/kg body weight highly decreased the locomotor activity of the test animals. In the hole board experiment ethyl acetate at dose of 200 and methanol at dose of 400 mg/kg body weight showed to some extent anxiolytic activity with compare to positive control grope. On the other hand nhexane fractions and methanol at dose of 200 mg/kg body weight did not showed any anxiolytic activity. On the other hand for elevated plus maze test, methanol and ethyl acetate soluble fraction may have anxiolytic activity. Whereas, n-hexane at dose of 400 mg/kg body weight did not have any anxiolytic activity.