IN VITRO PLANT REGENERATION OF LENTIL (Lens culinaris Medik.) FROM DIFFERENT EXPLANTS

Abstract

The investigation was carried out in the Tissue Culture Laboratory of the Department of Genetics and Plant Breeding, Hajee Mohammad Danesh Science and Technology University, Dinajpur during November, 2009 to April, 2010 with a view to study in vitro regeneration of Lentil (Lens culinaris Medik) from leaf discs and nodal segments explants. For this purpose, three varieties of Lentil viz. BARI Masur -3, BARI Masur -4 and BARI Masur -5 were used to assess their regeneration ability. Leaf disc and nodal segments of the three genotypes of Lentil were cultured on MS medium with different concentrations and combinations of growth regulators. Among the three varieties, BARI Masur -4 showed early callusing (17.67 days) with maximum rate of callus induction (83.33%) incase of leaf disc culture and also showed highest percentage of callus induction (80.55%) with minimum days (17.33 days) with nodal segment as_ explant. Early and maximum rate of callusing appeared in T, (MS +1.5 mg/L 2, 4-D + 0.5 mg/L BAP) and T; (MS +2 mg/L 2, 4-D + 0.5 mg/L NAA) from leaf disc and nodal segment respectively. BARI Masur -4 had the highest percentage of shoot regeneration from leaf disc (69.44%) and nodal segment (66.66%) explant. Early and maximum rate of regeneration was found in T, (MS+2.0 mg/L Kn + 0.2 mg/L NAA) and T3 (MS medium +0.5 mg/L BAP+ 0.25 mg/L Kn). The highest number of roots per shoot was counted in BARI Masur -4 (47.23%), in T; (MS medium containing 10mg/L IAA) and T, (MS medium containing 15mg/L IBA) considering leaf disc and nodal segment as explant. Considering the overall performance, genotype BARI Masur -4 appeared the best for callus formation, shoot regeneration and root formation.