ISOLATION AND IDENTIFICATION OF ORAL BACTERIA CAUSING PERIODONTITIS IN HUMAN WITH ANTIBIOGRAM STUDY

Abstract

The Presence study was conducted to isolate and identify the oral bacteria causing periodontitis in human by using morphological, cultural and biochemical techniques. However the prevalence of periodontitis was higher in young (65%) in comparing with child(1.3%) and adult(29%) stages. The prevalence of oral bacteria was higher in plaque in comperison with caries (27%) and tartar (23%) respectively. On the other hand the prevalence was also varied with the sugar consumption. In which the highest prevalence were absence in high sugar intake patients (30%) and lowest in less sugar intake patients (25.3%). Out of 150 samples 85% samples were found to be positive for Streptococcus and 75% for Staphylococcus and 65% for Porphyromonus gingivalis by using morphological and biochemical techniques. The identified isolates were also subjected to antibiogram study. In which most of the organism were resistant to maximum antibiotic which are available in commercially and sensitive to Ofloxacine. In this study it was observed that most of the oral bacteria were resistant to commonly used antibiotics that is the related for treating the oral infection on the other hand ofloxacine was only the sensitive antibiotic against oral bacteria. From this study it was concluded that determination of antibiotic susceptibility is very important in the determination of first line of choice for drugs in clinical control of periodontitis.