Study of Antimicrobial, Antioxidant and Thrombolytic activity of the fruit skin of Spondias pinnata

Abstract

The skin of Spondias pinnata fruit (family - Anacardiaceae) was subjected to clinical and biological examination in the study. The powdered whole plant was extracted with ethanol at room temperature (cold extraction) and the extract was partitioned between nhexane, dichloromethane,and Ethyl acetate fractions. The crude ethanoic extract and fractions were preliminary screened for their various phytochemical and pharmacological properties. The antimicrobial activities of extracts were examined. The crude extract produced strong activity against most of the organisms that’s why fraction of the crude extract was subjected to the antimicrobial test. The zones of inhibition produced by the n-hexane, dichloromethane, ethyl acetate and aqueous soluble partitionates of the ehanolic extract were ranged from 0-15 mm, 7-14 mm, 0-17 mm and 0-12 mm respectively at a concentration of 400μg/disc. Antioxidant property of the various fraction of the plant was determined by using five methods and good antioxidant activity was found for all the fractions. The study examined the thrombolytic potential of different extracts of S. pinnata skin in vitro. It is found that among all the samples only n-hexane fraction of the ethanolic extract of the plant has thrombolytic activity compared to the standard. Again, only single concentrations of the extracts were examined. So, finding of the dose related trend, if any, of thrombolytic activity can be subjected to further study.