Determination of Safrole and Isosafrole from soft drinks using Gas Chromatogram

Abstract

The aim and objective of this study is to determine the presence and concentration of safrole and isosafrole in different commercially available soft drinks to ensure the good health and safety of our life. Gas chromatogram technique was used to determine the presence of those compounds and measure their concentration. For this reason, a simple and rapid method was developed to determine the safrole and isosafrole in soft drink using a semi-polar column (RTX - 625) with direct injection gas chromatography. Direct quantitative analysis of safrole and isosafrole in soft drinks was carried out to determine their presence and concentration. The water soluble compound 1, 4-dihydroxybenzene (DHB) was used as the internal standard. Seven commercial soft drinks were analyzed by the current method, and results indicated that these seven commercial soft drink samples contained safrole, 0.39-.55 μg/ml safrole was detected, and most of them contained high level of safrole. Isosafrole was unable to detected using this method probably for its presence as derivatives.