ISOLATION AND IDENTIFICATION OF PATHOGENIC BACTERIA FROM SONALI CHICKEN MEAT AND STUDY OF THEIR ANTIBIOTIC SENSITIVITY PATTERN

Abstract

The present research work was conducted with a view to isolation and identification of pathogenic bacteria from sonali chicken raw meat and study of their antibiotic sensitivity pattern. The sample were collected aseptically from local market of Dinajpur city in Bangladesh and brought to the bacteriology laboratory, Department of Microbiology, HSTU, Dinajpur, during the period from July to December, 2017. A total of 50 samples were collected from the breast and thigh muscle of dressed sonali chicken. All of them were found to be contaminated with different microbes isolated as Salmonella spp. (100%), Escherichia coli (100%), Vibrio spp. (10%), and Staphylococcus spp. (92%). Isolated bacteria were identified using the biochemical tests namely Indole test, Methyl red test, Voges - Proskauer test, Citrate utilization test, Triple sugar iron test and MIU test. The study of antibiotic sensitivity pattern showed a number of pathogenic isolates were drug-resistant. All of the isolates were 100% sensitive to Gentamycin. It may be concluded from the study that, the sonali chicken meat usually contaminated with some bacteria. Therefore, it is suggested that, proper hygiene and sanitary practices should be maintained to make sonali chicken meat safe.