Mass Propagation and Conservation of Three Important Medicinal Plants: Aloe Vera L., Acorus Calamus L. and Stevia Rebaudiana Bertoni

Abstract

The peasant investigation was undertaken to standardize in vitro culture techniques for mass propagation and conservation of two threatened important plant species, Aloe vera L. and Acorus calamus L. of Bangladesh and one n: canty introduced non-sugar natural herbal sweetener, Stevia Reba Diana Bertoni. In case of Aloe vera through proper hormonal treatments it was possible to differentiate direct multiple shoots from shoot tip explants. The best multiple shoot production was achieved in MS+2.0 mg/I BA + 0.5 mg/I Kn + 0.2 mg/I NAA. The optimum pH level of the medium was found to be 6.0. As a carbon source, 13DH was the best and local market 13angladesh sugar at 30-40 g/1 was also found to give the satisfactory result. Agar at a level of 7 g/1 irrespective of brands was highly effective to solidly media. For adventitious rooting of regenerated shoots MMS1 + 0.2 mg/I NAA was proved to be the most favorable. The regenerated plants under ex vitro condition showed normal mode of development and 70% survivalist was achieved when they were transferred to garden soil + compost potting………