ORGANIC MANAGEMENT OF WILT COMPLEX AND TOMATO YELLOW LEAF CURL VIRUS IN SUMMER TOMATO USING BIO-AGENTS, BOTANICAL AND AGRO BIO-PRODUCTS FORTIFIED SOIL

Abstract

A pot and a lab experiment was conducted to evaluate the efficacy of selected bioagents

(Metarhizium anisopliae, Trichoderma harzianum, Verticillum lecanii, Beauveria bessiana), botanicals [Clerodendrum infortunatum (Vat) leaf extract, Lantana camara leaf extract, Azadiracta indica (Neem) leaf extract] and agro bioproducts (Tea wastage, Garlic powder, Mustard oil cake) against the wilt complex

and

Tomato yellow leaf curl virus (TYLCV) in summer tomato cultivation. The experiment was conducted at net house in the Department of Plant Pathology, Shere-Bangla Agricultural University (SAU), Dhaka, Bangladesh, during the period of

July to October, 2021. The experiment was carried out in a complete randomized

design (CRD) with three replications and each of the treatment contains 3 pots. The

total number of unit pots was 30. Summer variety ‘BARI hybrid tomato-4’ was used

as a tested cultivar. Data on disease incidence and disease severity of wilt complex

and TYLCV was recorded at 30, 45 and 60 DAT. Fungal pathogen was isolated by

tissue planting

method. Bacterial pathogen was isolated by dilution and streaking method. In case of wilt complex, among the selected bio–agents, the lowest disease incidence (33.33%) and severity (15%) was found in T 1 (Metarhizium anisopliae + Trichoderma harzianum) and the highest disease incidence (66.67%) and severity (20%) was found in T 3 (Metarhizium anisopliae + Beauveria bessiana). In case of TYLCV, the lowest disease incidence (66.67%) and severity (20%) was found in T 3

(Metarhizium anisopliae + Beauveria bessiana) and the highest disease incidence (100%) and severity (30%) was found in T 2 (Metarhizium anisopliae + Verticillum lecanii). From the present study, it was found that the wilt complex of tomato caused by Fusarium oxysporum f.sp. lycopersici and Ralstonia solanacearum that was confirmed by morphological characteristics of macro and micro conidial structures and biochemical tests viz. gram staining, motility, KOH solubility, catalase and simon’s citrate test, respectively. The performance of the treatments in respect of yield and yield contributing characters against wilt complex and TYLCV of summer tomato varied significantly. However, from the present study it may be concluded that mustard oil cake and neem leaf extract can be used as ecofriendly approach for effective management of wilt complex and Tomato Yellow Leaf curl Virus (TYLCV) in summer tomato cultivation.