Study of Microbial Load and Their Antibiotic Resistance in Different Samples of Ostrich

Abstract

The study was conducted to monitor the microbial assessment in oropharyngeal swab , cloacal swab and fecal samples of ostrich and also update knowledge on antimicrobial sensitivity of isolated organisms .The study was performed at HSTU ostrich farm from 16th January to 15th June, 2016. A total 75 samples were collected from 8 ostriches at different age of which 25 oropharyngeal, 25 cloacal swabs sample and 25 were feces samples. The organisms were isolated by using standard microbiological method. The result revealed that the average microbial load in plate count agar was highest in feces (1.48 ×109±0.27CFU) than oropharyngeal swab(1.36×109 ±0.38 CFU) and cloacal swab (1.28×109 ±0.34 CFU) .Among seventy five sampls, 29 (38.66%) Escherichia coli, 21 (28%) Salmonella spp, 20 (26.67%) Staphylococcus spp. 5 (6.6%) Bacillus spp. were identified. In twenty five oropharyngeal swab sampls, Staphylococcus spp was found higher 15 (60%) than other organisms but Escherichia coli were found higher both in cloacal 11(44%) and faecal 12(48%) samples. On antibiogram study Escherichia coli, Salmonella spp, Staphylococcus spp. and Bacillus spp were more sensitive to Levovidine, Azithromycin, Nalidixic Acid and Amoxicillin respectively while more resistant to Azithromycin, Penicillin ,Chloramphenicol and Erythromycin respectively . The obtained results indicated that ostrich excreta is one of the most important sources of Escherichia coli, Staphylococcus spp, Salmonella spp, Bacillus spp.in ostrich farms. Finally, it may be concludes that the logical use of antibiotics must be adopted in ostrich farms reared in Bangladesh for prevention the appearance of multi drug resistance bacteria. Moreover proper measures should be taken to ward off zoonotic diseases in peoples who are related to ostriche farming