ISOLATION AND IDENTIFICATION OF BACTERIA FROM BETEL LEAF WITH THEIR ANTIBIOTIC RESISTANCE PATTERN

Abstract

The experiment was carried out to isolate and identify bacteria as well as to detect antibiotic sensitivity pattern of isolated bacteria from infected betel leaf sold at local markets of Dinajpur city, Bangladesh. For this study, a total 20 infected betel vine sample were collected from five different local markets (Leili morr, Terminal road, Doshmile, Gopalgong bazaar, and Basharat) of Dinajpur. A series of test were conducted for isolation, identification and frequency distribution of different bacteria of betel leaf. A total of 20 bacterial isolates belong to five genera (staphylococcus spp., Bacillus, spp., Escherichia coli, klebsiella spp and Entrobacter spp.) were identified. Out of 20 samples, 6 were staphylococcus spp (30%), 4 were Bacillus spp. (20%), 1 were Escherichia coli (5%), 5 were klebsiella spp. (25%) and 4 were Entrobacter spp. (20%). The identified isolation were subjected to antibiogram study in which staphylococcus spp. (6) were sensitive to Gentamicin (100%), followed by ciprofloxacin (83.33%), Vancomycin (66.66%), Erythromycin (33.33%), and resistant to Kanamycin (83.33%), Bacillus spp. (4) were found sensitive to Erythromycin (100%), were sensitive to followed by ciprofloxacin (75%), Neomycin (75%), Co-trimoxazole (50%), and resistant to Amoxicillin (100%). E.coli spp. (1), were found sensitive to Ciprofloxacin (100%), Co-trimoxazole (100%), Neomycin (100%). and resistant to Erythromycin (100%), and Amoxicillin (100%). klebsiella spp. (5), were found sensitive to Ciprofloxacin (100%), were sensitive to followed by Co-trimoxazole (80%), Neomycin (80%), and resistant to Amoxicillin (100%), Erythromycin (100%). Entrobacter spp. (4), were found sensitive to ciprofloxacin (100%), were sensitive to followed by kanamycin (75%), and resistant to Amoxicillin (100%), Ampicillin (100%), and Cefuroxime Sodium (100%). So Antibiogram result indicated the ciprofloxacin, Co-trimoxazole and Gentamycin, in optimum doses would be the drug of choice to treat the most cases of human infection caused by consumption of infected betel leafs.