Review on simultaneous estimation of fixed dose combination: system suitability parameter of RP-HPLC analysis

Abstract

In modern pharmaceuticals, Reverse Phase- High performance Liquid Chromatography (RP- HPLC) is one of the most essential tools. When analyzing, attempting to separate, and

identifying compounds from a complicated mixture, RP-HPLC is the most advantageous option because it permits purification of most classes of compound. For any chromatography system suitability is required and are designed to assess the analytical system's constituent parts and demonstrate that its performance satisfies the criteria set forth by the method. 12 articles were picked from number of reliable sources and journals where RP-HPLC was used to asses combination drugs in solid dosage form. In this review article, the system suitability of those selected studies is reviewed on the basis of USP guideline in order to criticize and evaluate the robustness of the method. If any incomplete or misleading information was identified, the possible cause and solution for those methodologies were explained in this review.