ISOLATION, IDENTIFICATION AND ANTIBIOGRAM STUDY OF BACTERIA CAUSING GOAT DIARRHOEA IN KURIGRAM DISTRICT

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2014-12

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HAJEE MOHAMMOD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR

Abstract

The present research was conducted for the isolation and identification of the bacterial pathogen causing goat diarrhoea in Kurigram district and antibiogram study for appropriate treatment of causal agent. For this purpose 70 faecal samples were collected directly from the rectum of diarrhoeic goat during the period of July, 2014 to December, 2014 brought for the treatment in different veterinary hospitals under Kurigram district. Of the 70 experimented faecal samples, Salmonella spp. 21(30.0%), Escherichia coli 38 (54.3%), were identified as single or mixed infection and 11(15.7%) diarrhoea were found non-specific infection. Among 59 infected samples 9 (15.25%) were identified as mixed infection of Salmonella spp. and Escherichia coli. \solation and identification of the bacteria were confirmed on the basis of morphology, staining character, cultural and biochemical properties. Antibiotic sensitivity study revealed that FE. coli organism from goats diarrhea were highly sensitive to Kanamycin, Chloramphenicol and Flumequine, moderately sensitive to Pefloxacin, Amikacin, less sensitive to Ceftriaexone, Colistin Sulphate, Cephradine and Bacitracin, while resistance to Amoxycillin/ Clavulanic acid, Penicillin G & Sulphamethoxazole/ Trimethoprim. Among the isolates Salmonella sp were highly sensitive to Amikacin, Kanamycin and Chloramphenicol, moderately sensitive to Flumequine Colistin Sulphate and Pefloxacin but less sensitive to Ceftriaexone, Sulphamethoxazole and Cephradine. Besides showed resistant to Amoxycillin, Penicillin G & Bacitracin. The findings of the experiment indicated that Kanamycin, Chloramphenicol and Amikacin may be used for the clinical control of E. coli and Salmonella spp. causing goat diarrhoea in Kurigram district.

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Goat rearing has become seriously impaired due to high mortality with diarrhoea like symptoms. The marginal and land-less farmers most easily live on rearing of goats in Bangladesh. So, goat is called the poor man's cow that is the second important livestock in Bangladesh which plays an important role in the rural economy and we can earn substantial amount of foreign currency by exporting skin and other byproducts.

Keywords

KURIGRAM DISTRICT, Solid Culture Media, Nutrient Broth (NB), MacConkey (MC) Agar, Eosin Methylene Blue (EMB) Agar

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