ISOLATION, IDENTIFICATION AND ANTIBIOGRAM STUDY OF BACTERIA CAUSING GOAT DIARRHOEA IN KURIGRAM DISTRICT
Date
2014-12
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
HAJEE MOHAMMOD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR
Abstract
The present research was conducted for the isolation and identification of
the bacterial pathogen causing goat diarrhoea in Kurigram district and
antibiogram study for appropriate treatment of causal agent. For this
purpose 70 faecal samples were collected directly from the rectum of
diarrhoeic goat during the period of July, 2014 to December, 2014 brought
for the treatment in different veterinary hospitals under Kurigram district.
Of the 70 experimented faecal samples, Salmonella spp. 21(30.0%),
Escherichia coli 38 (54.3%), were identified as single or mixed infection
and 11(15.7%) diarrhoea were found non-specific infection. Among 59
infected samples 9 (15.25%) were identified as mixed infection of
Salmonella spp. and Escherichia coli. \solation and identification of the
bacteria were confirmed on the basis of morphology, staining character,
cultural and biochemical properties. Antibiotic sensitivity study revealed
that FE. coli organism from goats diarrhea were highly sensitive to
Kanamycin, Chloramphenicol and Flumequine, moderately sensitive to
Pefloxacin, Amikacin, less sensitive to Ceftriaexone, Colistin Sulphate,
Cephradine and Bacitracin, while resistance to Amoxycillin/ Clavulanic
acid, Penicillin G & Sulphamethoxazole/ Trimethoprim. Among the isolates
Salmonella sp were highly sensitive to Amikacin, Kanamycin and
Chloramphenicol, moderately sensitive to Flumequine Colistin Sulphate and
Pefloxacin but less sensitive to Ceftriaexone, Sulphamethoxazole and
Cephradine. Besides showed resistant to Amoxycillin, Penicillin G &
Bacitracin. The findings of the experiment indicated that Kanamycin,
Chloramphenicol and Amikacin may be used for the clinical control of E.
coli and Salmonella spp. causing goat diarrhoea in Kurigram district.
Description
Goat rearing has become seriously impaired due to high mortality with
diarrhoea like symptoms. The marginal and land-less farmers most easily
live on rearing of goats in Bangladesh. So, goat is called the poor man's cow
that is the second important livestock in Bangladesh which plays an
important role in the rural economy and we can earn substantial amount of
foreign currency by exporting skin and other byproducts.
Keywords
KURIGRAM DISTRICT, Solid Culture Media, Nutrient Broth (NB), MacConkey (MC) Agar, Eosin Methylene Blue (EMB) Agar
