DETECTION OF SALMONELLA SPP. FROM POULTRY PRODUCTION CHAIN IN DINAJPUR WITH ANTIBIOTIC SENSITIVITY TEST

Abstract

Bangladesh is an agriculture based country. Poultry rearing is considered superior to the others in agricultural sector. Salmonella spp. is one of the most economically significant organism in poultry sector. The aim of this study was to identify and characterize Salmonella species isolated from poultry production chains of Dinajpur (Kaharol and Sadar Upazila) district of Bangladesh during the period of January to June 2016. For this purpose a total of 153 samples (chick meconium, cloacal swab, poultry carcass, feed, water, transport swab and floor swab) were collected and were subjected to various cultural and biochemical techniques. Furthermore, the isolated Salmonella species were characterized by antimicrobial susceptibility testing. Among the samples, 23.53% (n=36) were found to be associated with Salmonella species. The Salmonella species were identified by observing on SS agar, positive to MR test and negative to VP and Indole test. Among the 36 isolates, 30.56% (n=11) belonged to serogroup B and rest of the isolates 69.44% (n=25) to serogroup D. The isolated Salmonella species were subjected to antimicrobial susceptibility testing with the aid of disk diffusion method using 8 antimicrobial agents. All isolates of Salmonella species were susceptible to ciprofloxacin, norfloxacin, streptomycin and gentamicin. Out of 36 isolates 100% Salmonella species were resistant to erythromycin and tetracycline. The findings of this study revealed the presence of multidrug resistant Salmonella species in poultry production chains of Dinajpur (Kaharol and Sadar Upazila) district of Bangladesh that posseses a serious threat to public and poultry health.