GENETIC DIVERSITY OF LEMON GENOTYPES ASSESSED BY ISOZYMES

Abstract

An experiment was carried out at the department of Horticulture, Bangladesh Agricultural University, Mymensingh to investigate genetic diversity of 73 lemon genotypes using three isozymes viz, glutamate oxaloacetate transminase (GOT), malate dehydrogenase (MDH) and peroxidase (PER) isozymes during 2006-2007. All the isozymes, used in the present study showed polymorphism for lemon. A total of 25 different electrophoretic zymotypes were observed for three isozymes studied. Eight zymotypes with GOT, 10 with MDH and 7 with PER were formed by 22, 39, 12 bands at different Rf values. respectively. A dandogram was constructed and 73 lemon genotypes were grouped into nine clusters, the genotypes collected from same location were grouped in different clusters, indicating existance of variability within single location. The result revealed that isozymes are useful genetic marker for variability analysis of lemon and other species of Citrus.