In vitro propagation of Stevia rebaudiana

Abstract

The experiment was conducted at the Plant Tissue Culture Center Trust Laboratory of Proshika Plant Tissue Culture Trust (PPTCC), Koitta. Manikgonj, Bangladesh from October, 2007 to June. 2008. For shoot proliferation, MS (Murashige and Skoog) and White's media supplented with four PGRs (BAP,Kn, NAA,ADS) were used singly at five concentrations (0.1, 1.0, 1.5, 2.0, 2.5 mgfL). BA? 2mg/L and Kn 1 and 2 mg/L were used in MS and White's media for synergism. PGRs and organic supplement viz., BAP, Kn, NAA, lB A, IAA and orange and tomato juice were added in Linsmiaier and Skoog (LS) and Phytamax (PM) media For callus induction, 2,4-D 2mgIL was added in MS and White's media .For root formation, MS, LS, PM and White's media with IBA 2mgIL was used. For plantlet etablishment. the proportion of soil, sand and fertilizer /cowdung/poultry litter were used in 1:1 :1. MS media with 2.5 mg/L was the best shoot proliferation using single PGR For, combined etTect of PGR and organic supplement, best result was found with PM media supplented with IBA 0.5 mg/L. For synergism, best result was found with MS media supplemented with BA? mg/L with Kn I mg/L. Maximum root formation was observed with MS media with IBA I mgfL. MS media with 2,4-D 2mgfL was the best for callus induction. Combination of garden soil, sand and cowdung (1:1:1) was the best plantlet establishment comparing other treatments.