BACTERIOLOGICAL ANALYSIS WITH ANTIBIOTIC RESISTANCE PATTERN OF BACTERIA ISOLATED FROM PRAWNS AT DINAJPUR, BANGLADESH

Abstract

Prawns sold in local market could be microbiologically spoiled intrinsically or extrinsically. The research study was attempted to detect Total Bacterial Count (TBC), Total Coliform Counts (TCC), Total Salmonella-Shigella (TSS) Counts and Total Vibrio Counts (TVC). The abundance of bacterial loads was determined from thirty prawn samples. The density of total bacteria was detected in all the prawn samples were significantly higher (p< 0.001). Mean±SEM of TBC, TCC, TSS and TVC of prawn samples was 4.49±0.24, 5.20±0.29, 4.60±0.30 and 5.59±0.21 log10 CFU/g respectively. The total bacterial counts (TBC) ranged between 3.8×102 CFU/g to 2.8×106 CFU/g. The total coliforms counts (TCC) ranged between 4.9×102 CFU/g to 3.52×107 CFU/g. Furthermore, total Salmonella-Shigella (TSS) counts ranged from 1.65×102 CFU/g to 1.3×107 CFU/g. The total Vibrio counts (TVC) of thirty samples were ranged between 4.0×103 CFU/g to 2.5×107 CFU/g. The highest Mean±SEM of TBC, TCC, TSS and TVC were found in Gopalgong bazaar (4.76±0.39 log10 CFU/g), Rail gate bazaar (6.13±0.41 log10 CFU/g), Rail gate bazaar (5.79±0.32 log10 CFU/g) and Rail gate bazaar (5.29±0.40 log10 CFU/g) respectively. But the bacterial load found in prawns did not vary significantly among the three selected local markets of Dinajpur city. A total of thirty prawn samples were examined. All of them were found to be contaminated with different microbes characterized as follows- E. coli (100%), Salmonella spp. (100%), Vibrio spp. (66.7%), Klebsiella spp. (40%) and Shigella spp. (33.3%). Isolated bacteria were identified using the biochemical tests namely Indole test, Methyl red test, Voges - Proskauer test, Citrate utilization test, Triple sugar vi Iron test and MIU test. The results revealed that the bacterial load found to be higher in most of the samples. The study of antibiotic resistance pattern showed a number of pathogenic isolates to be drug-resistant. All of the isolates were found to be 100% resistant against Tetracycline. E. coli and Vibrio spp. also showed 100% resistant against Cefixime and Erythromycin respectively. Key words- Prawn, Total Bacterial Count (TBC), Total Coliform Count (TCC), Total Salmonella-Shigella (TSS) Count, Total Vibrio Counts (TVC), Antibiotic resistance.