PREVALENCE AND ANTIBIOGRAM ASSAY OF ZOONOTIC BACTERIA IN RAW MEAT

Abstract

The present research work was undertaken to isolate and to investigate the prevalence of zoonotic bacteria from broiler meat samples sold in Krishi Market, Bihari Camp, Agargaon Bazar, Taltola Bazaar and SAU Mini Bazaar, Dhaka, Bangladesh from January to May 2018. A total of 45 samples were randomly collected from markets and transported to the Microbiology and Parasitology laboratory of Sher-e-Bangla Agricultural University for microbiological analysis. After processing of samples primary culture was done in nutrient broth and nutrient agar then pure culture was obtained from different selective media. The prevalence of Escherichia coli, Salmonella spp. and Staphylococcus spp. were 74%, 42% and 18% respectively. All pure isolates were subjected to Antibiogram assay test by disc diffusion method against 8 different antibiotics. E. coli isolates were sensitive to ciprofloxacin (91.6%), gentamycin (87.5%), azithromycin (66.66%), tetracycline (58%) and resistant to penicillin (79.16%) , amoxycillin (75%), streptomycin (75%) and ampicillin (58.3%). Among all Salmonella spp. isolates, ciprofloxacin (81.81%) showed the highest susceptibility pattern followed by the gentamycin (72.72%) and azithromycin (63.63%). Highest resistant pattern was showed by tetracycline (58%), streptomycin(72.72%), penicillin (72.72%) and amoxycillin (63.63%). In case of Staphylococcus spp., ciprofloxacin (85.71%) showed the highest susceptibility pattern followed by the gentamycin (71.42%). also, ampicillin (71.42%), streptomycin (57.14%), azithromycin (57.14%) and tetracycline (57.14%) found sensitive in this study. Highest resistant pattern showed by amoxycillin (71.42%) and penicillin (71.42%). This study revealed that broiler meat sold at some local markets in Dhaka city were contaminated with multiple species of multidrug resistant bacteria which may risk for human health.