BACTERIOLOGICAL ASSESSMENT OF HOUSEHOLD AND POTABLE WATER SAMPLES IN HSTU DINING-HALL

Abstract

The research work was conducted in order to isolate, identify, characterize and to detect antibiotic resistance pattern of Salmonella spp., E. coli, Klebsiella spp., Staphylococcus spp. and Shigella spp. found in the household water and potable water. A total of 50 samples were collected from different dining-halls of HSTU, Dinajpur district and brought to the Microbiology laboratory of Hajee Mohammad Danesh Science and Technology University for bacteriological examination. The isolates were detected by standard bacteriological tests including cultural tests, staining and biochemical tests. The study revealed that the prevalence of E. coli, Salmonella spp., Klebsiella spp., Shigella spp. and Staphylococcus spp. in household water was 20%, 44%, 16%, 8% and 12% respectively, on the other hand in potable water it was 28%, 32%, 12%, 8% and 20% respectively. The total prevalence of E. coli, Salmonella spp., Klebsiella spp., Shigella spp. and Staphylococcus spp. in household water and in potable water was 24%, 38%, 14%, 8% and 16% respectively. The results also suggest that both household and potable water pose significant alarming for the public health issue if not maintain proper hygienic steps in place. The study of antibiotic resistance pattern showed a number of pathogenic isolates to be drug resistant and drug sensitive. Isolates of Salmonella spp., E. coli and Klebsiella spp. were found sensitive to Colistin (100%). Salmonella spp., E. coli, Klebsiella spp. and Shigella spp. found resistant to Erythromycin (100%) while Staphylococcus spp. found intermediate to Erythromycin (62.5%). So it can be suggested that the antibiotic resistance properties of isolated bacteria can cause serious health hazards because of ineffective treatment of the sufferers by the commonly prescribed antibiotics.