ISOLATION AND IDENTIFICATION OF BACTERIAL PATHOGENS FROM URINARY TRACT INFECTION IN HUMAN

Abstract

The aim of the study was to diagnose the type of pathogens resposible for urinary tract infection (UTI) and test their sensitivity to different antibiotics that help the physician to choose the correct empirical treatment. Urine samples were 6 cultured on Nutrient agar, MacConkey agar, Mannitol Salt Agar, Blood agar, EMB Agar, Cetrimide Agar and Staphylococcus Agar No. 110 plates with overnight incubation. Identification of organisms was done by conventional methods. The sensitivity test was carried out on Nutrient agar, Mueller-Hinton agar plates unless otherwise stated. A total of 58 clinical samples, 16 (27.6 %) were male of which 6 (37.5%) were positive and that of 42 (72.4 %) were female of which 19 (45.2%) were positive for UTI. From total collected samples, 43.1 % were culture positive. The predominant organisms were Escherichia coli, Klebsiella spp., Pseudomonas spp. and Staphylococcus spp. E. coli showed sensitivity to Ciprofloxacin 73.3%, Chloramphenicol 100%, Gentamycin 80% and Amikacin 80% respectively. Klebsiella spp. showed sensitivity to Ciprofloxacin 50%, Gentamicin 100%, Amikacin 50% and Chloramphenicol 50%. Pseudumonas spp. showed sensitivity to Ciprofloxacin 100%, Gentamicin 100% and Livofloxacin 100%. Where as Staphylococcus spp. showed sensitivity to Ciprofloxacin 100%, Gentamicin 85.7%, Chloramphenicol 100% and Amikacin 51.1%. It is concluded that females had more UTI than that of males specially, at the age of 31-45 years. More than 50% of the predominant isolates were sensitive to Chloramphenicol, Gentamicin, Ciprofloxacin and Amikacin.