IN VITRO REGENERATION OF LISIANTHUS (Eustoma grandiflorum (Raf.) Shinn)

Abstract

An experiment was jointly accomplished at Sher-e-Bangla Agricultural University and ASRBC, ACI, Dhaka, Bangladesh to develop a protocol for in vitro regeneration of Lisianthus during the period from January, 2015 to September, 2016. Response of leaf explants different hormonal concentrations in different media were observed. Initiation of callus and response towards shooting were earlier in MS medium than B5 medium with 1.5 mg/l NAA supplementation. In case of shoot regeneration, four combinations of BAP and GA 3 (0.5X1.0, 1.0X0.5, 1.5X1.0, 1.0X1.5 mg/l respectively) were used. Highest mean number of shoot (16.3) and shoot length (2.6cm) were recorded when supplemented with 1 mg/l BAP and 1.5 mg/l of GA 3 on MS medium. Full strength MS, half strength MS and B5 media supplemented with different concentrations of IBA and IAA were used for root initiation, where 1.5 mg/l of IBA was found to be the best for rooting. In vitro regenerated plantlets were acclimatized in sand, vermicompost and coco dust with the ratio of 1:1:1.