ISOLATION AND IDENTIFICATION OF BACTERIA FROM ENVIRONMENTAL SAMPLE OF LIVE BIRD MARKET AND THEIR ANTIBIOGRAM STUDY ABDIRAHMAN AHMED MOHAMED

Abstract

The research work was conducted in order to isolate, identify, characterize and to detect antibiotic resistance pattern of Salmonella spp., E. coli, Klebsiella spp. and Shigella spp. found in the environmental samples. A total of 30 environmental samples were collected from live bird market of Dinajpur district and brought to the Microbiology laboratory of Hajee Mohammad Danesh Science and Technology University for bacteriological examination. The isolates were detected by standard bacteriological tests including cultural tests, staining and biochemical tests. The study revealed that the total prevalence of E. coli, Salmonella spp., Klebsiella spp. and Shigella spp. in environmental samples were 26.7%, 33.3%, 16.7% and 23.3% respectively. The results also suggest that total samples pose significant alarming for the public health issue if not maintain proper hygienic steps in place. The study of antibiotic resistance pattern showed a number of pathogenic isolates to be drug resistant and drug sensitive. Antibiotic sensitivity test revealed that Salmonella spp., E. coli and Shigella spp. were sensitive to Chloramphenicol (100%) while Klebsiella spp. was resistant to Chloramphenicol (100%). E. coli, Klebsiella spp. and Shigella spp. were resistant to Erythromycin (100%) while Salmonella spp. was found intermediate to Erythromycin (60%). So it can be suggested that the antibiotic resistance properties of isolated bacteria can cause serious health hazards because of ineffective treatment of the sufferers by the commonly prescribed antibiotics