BACTERIOLOGICAL ANALYSIS AND MOLECULAR CHARACTERIZATION OF RAW MEAT OF GOAT

Abstract

Goat farming is one of the important components of the livestock industry in Bangladesh. Farmers rear goats mostly as a subordinate occupation to supplement their livelihood. Goat farming is not only a commercial enterprise but also a mode of life which contributes substantially in farmer’s income as well as in national economy. This study was carried out to investigate the bacteriological quality of raw meat of goat selling in different markets at sadar Upazila in dinajpur, Bangladesh. A total of 30 meat samples were collected from Dinajpur district of Bangladesh and tested for the presence of microorganisms following conventional microbiological process. Samples were inoculated into various selective media such as manital salt (MS) agar, Eosine Methylene Blue (EMB) agar, Nutrient agar, Mac conkey agar, Salmonella- Shigella (SS) agar, Staphylococcus agar no. 110. Identification of Bacteria were performed by morphological, cultural and biochemical test. The total viable count (TVC) in meat samples of Bahadur bazar was 4.8 CFU/g, 4.6 CFU/g and 3.9 CFU/g, in 10-3 , 10-7 and 10-10 dilution respectively and Borobondor bazar was 3.6 CFU/g, 3.2 CFU/g and 3.02 CFU/g in 10-3 , 10-7 and 10-10 dilution respectively. The isolated staphylococcus spp. was sensitive to Chloramphenicol (C), Ciprofloxacin (CIP) and Kanamycm (K), Salmonella spp. was sensitive to Azithromycin (AZM), Vancomycin (VA) and Teicoplanin (TE) and Pseudomonas spp. was sensitive to Chloramphenicol (C), Ciprofloxacin (CIP). The identified isolates were characterized by using molecular technique like PCR, DNA sequencing and phylogenetic Analysis. This study reveals that raw meat samples in Bahadur bazar are contaminated beyond the acceptable limits. This may be due to the unhygienic practices of butchers consumers were not concious. Suggestion has been given to improve the knowledge on hygienic among the meat producers to reduce microbial load.