CHARACTERIZATION OF STAPHYLOCOCCUS SP ISOLATED FROM ARTHRITIC BROILER BREEDER FLOCK WITH ANTIBIOGRAM STUDY OF IDENTIFIED FIELD ISOLATES

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2011-08

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HAJEE MOHAMMOD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR

Abstract

The study was conducted for characterization of Staphylococcus sp isolated from arthritic broiler breeder flock with antibiogram study of identified field isolates. The study was performed in a breeder flock. The flock consists of 7000 birds of different age groups, name of the breed cobb 500 selected for the study. The sample were collected from the affected birds and shifted aseptically to the Bacteriology laboratory of the department of Microbiology Hajee Mohammed Danesh Science and Technology University. The samples were subjected to clinical, postmortem and microbiological (morphological, cultural and biochemical) examination. The study shows that infection is very high in starter and grower (58.2%) than laying period (16.6%). The staphylococcus sp highly sensitive to different antimicrobial agents such as 100% sensitive to ciprofloxacin, doxycycline, penicillin, gentamicin and 70% sensitive nitrofurantoin 60% azithromicin and resistance to cephradine, ampicillin, amoxicillin sulfomithoxazole X Trimethoprim.

Description

The importance of Staphylococcosis in poultry sector has increased to be a growing concern day by day throughout the world during the last decades. Staphylocococcus aureus is a normal inhabitant of the skin and upper respiratory tract of diseased and healthy chickens (Shiozawa et al., 1980; Zhu et al., 1999). Several species of Staphylococcus cause infection in poultry. Staphylococcus aureus is an important opportunist that can cause superficial to life-threatening illnesses in a variety of animal species. In poultry, this organism has been implicated in osteomyelitis, synovitis, and cellulites (White DG, 2003).The Staphylococcus sp cause a number of infection in poultry such as arthritis, osteomyelitis, dermatitis, endocarditis, septicemia, wound infection, and ophthalmitis. Staphylococcus infections tend to occur more frequently during the following four periods of a breeder’s life: 0 - 2 weeks femoral head necrosis (or bacterial chondronecrosis) are often related to egg or hatchery contamination and minor surgeries at, 4 - 6 weeks infection hock and stifle joints secondarily related to other infection, at 10 - 20 weeks Infection hock and stifle joints is due to the stress of vaccination, feed restriction and sexual maturation. Overcrowding, poor feed distribution and insufficient feeder space exacerbate these problems.

Keywords

IDENTIFIED FIELD ISOLATES, Mannitol Salt Agar, Antibiotic Discs

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