PREVALENCE OF MASTITIS IN GOATS, ISOLATION OF ETIOLOGICAL BACTERIA AND IDENTIFICATION OF THEIR ANTIBIOTIC RESISTANCE PROPERTIES

Abstract

Mastitis is one of the multifactorial and expensive diseases of the dairy animals as it not only reduces the milk production but also leads to detrimental changes in the milk composition. The aim of this work was to determine the prevalence of clinical and subclinical caprine mastitis with their associated bacterial pathogens and antibiotic sensitivity patterns during the period from January to June, 2017. A total of 120 lactating goats in Dinajpur district were randomly selected for this study and 240 milk samples from each udder half were screened for evidence of clinical mastitis by clinical examination of udder and subclinical mastitis by CMT (California Mastitis Test). The overall prevalence of clinical and subclinical mastitis was found to be 11.67% and 38.75%, respectively. Bacteriological examination of mastitic milk samples revealed that Staphylococcus spp. (32.5%) was the major etiological agent of caprine mastitis followed by Escherichia coli (22.5%), Pseudomonas spp. (12.5%), Klebsiella spp. (12.5%), Bacillus spp. (5%). Statistical results of the study showed that parameters like age, parity, litter size and teat lesions had significant effect on caprine mastitis (p< 0.05) but lactation stage had insignificant effect on mastitis (p> 0.05). Antibiotic sensitivity test studies of mastitic pathogens from goat milk revealed sensitivity to Levofloxacin, Gentamicin, Ciprofloxacin and Chloramphenicol. Antibiotic resistance was seen against Penicillin, Amoxicillin, Azithromycin, Cloxacillin, Erythromycin, Vancomycin, Amikacin, Cefixime and Cephradine at various degree; the antibiotic resistance me be due to indiscriminate use of antibiotics in diseases control. It may be concluded that consumption of infected goat milk by children may host antibiotic resistance bacteria, the antibiotic resistance gene may transfer to the normal micro-flora that may lead super infection.