ISOLATION, IDENTIFICATION AND ANTIBIOGRAM STUDY OF BACTERIAL PATHOGENS FROM STORED WATER IN TANKS AT HSTU MALE HALLS

Abstract

The present study was conducted to isolate, identify and Antibiogram study bacteria from stored water in tanks of some selected Tanks at HSTU Male Halls in Dinajpur district of Bangladesh. total of 36 samples were collected Aseptically from different Tanks, During this period from August to November 2019. The stored water are collected and brought to the laboratory under the Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dinajpur the stored water different bacteriological tests for isolation allowed identification of bacteria based on morphology, staining, cultural and biochemical examinations and Antibiogram study. The study revealed that the total prevalence of bacterial pathogens isolated and identified from stored water samples were E. coli 11(30%) Salmonella spp 8(22%) Klebsiella spp 2(5.5% Shigella spp. 5(13.9%) Staphylococcus spp. 5(13.9%) and Pseudomonas spp. 2(5.5%) Vibrio spp (2.8%) Micrococcus 2 (5.5%) The Most effective antibiotics against Salmonella spp, Shigella spp, Pseudomonas spp, staphylococcus spp, Vibrio spp, micrococcus spp is Streptomycin, Chloramphenicol, Amikacin, moxifloxacin were Ampicilin, Amoxycillin and Tetracycline are Resistant In the context of this study, it may be concluded that: The presence of E. coli, Salmonella spp. Klebsiella spp., Shigella spp., Staphylococcus and Pseudomonas presents stored water in Tanks Wich Makes health hazards to both students and Other staffs to cause virous Disease such as diarrhea, dysentery, cholera, typhoid and gastroenteritis. The antibiotic resistance properties of isolated bacteria can cause serious health hazards because of ineffective treatment of the sufferer’s by the commonly described antibiotics.