Investigation of cytotoxicity and anti-microbial property of the methanolic extract of aphanamixis polystachya fruit

Abstract

The purpose of this study was to understand and investigate the cytotoxic and antimicrobial effect of the traditional medicinal plant Aphanamixis polystachya. Toxicologically it is classified under the family Meliaceae. In this paper the study was conveyed on the methanol extract of the fruit of Aphanamixis polystachya. Many researches were done on this plant species by many scholars at different time period but the intention of the study was to emphasize on the possibility of its cytotoxic property. To prove the cytotoxic property and detect its intensity, the Brine Shrimp Lethality Bio-assay and to measure the anti-microbial property Disk-diffusion method (Kirby-Bauer) was chosen. After collection, identification and drying the fruit sample, extraction was performed by using methanol solvent system. The extracted components were used to measure the cytotoxicity level over Brine Shrimp Nauplii and anti-microbial property was tested over Candida albicans, Shigella dysentery and Staphylococcus aureus.