ISOLATION AND IDENTIFICATION OF ENTEROBACTERIA FROM APPARENTLY HEALTHY, SICK AND DEAD BROILERS
Date
2012-06
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR.
Abstract
The present research work was conducted with a view to isolation and
identification of enterobacteria from apparently healthy, sick, and dead
broilers. The sample were collected from Thakurgaon and Dinajpur district
in Bangladesh and brought to the Department of Microbiology, Faculty of
Veterinary and Animal Science, Hajee Mohammad Danesh Science and
Technology University(HSTU), Dinajpur, during the period from January to
June, 2012. A total of 236 samples were collected from 6 flocks of 3 farms
and the collected samples were liver, cloacal swab and intestinal swab from
healthy, sick, and dead broilers respectively. The samples were composed of
95 healthy, 69 disease and 72 dead broilers and the isolated enterobacteria
were Escherichia coli , and Salmonella spp. Out of 236 samples
185samples were positive for both Escherichia coli (115) and Salmonella
spp.(70). The cultural prevalence of Escherichia coli in healthy broiler was
39.19 %, in sick broiler was 36.95 %, and in dead broiler was 58.14 %. The
prevalence of Salmonella spp. was 17.40%, 23.14% and 26.69 % in healthy,
sick and dead broilers respectively in this study. It was found that the
highest prevalence of Escherichia coli and Salmonella spp. was 58.14 %
and 26.69% respectively in case of dead broilers. It may be concluded from
this study that the enteric bacteria usually remain in the gastrointestinal tract
and as clinically over infection and do not produce clinical disease unless or
until other factors are involved.
Description
Bangladesh is one of the poorest countries of the third world in terms of
material resources. Its economy mainly depends on agriculture. Poultry
industry is an emerging agribusiness starting practically during eighties in
Bangladesh. Poultry rearing may play a vital role for the poverty alleviation.
About 80% of the total population of 140 million is living in the
68,000 villages of Bangladesh, and almost each and every village home
holds 6-7 chickens. It is estimated that there are about 153 million
chickens in Bangladesh. There are about one lac poultry farms in
Bangladesh, of which 20% rearing 1000 to 50,000 birds and remaining
80% are small in size with 100 to 1000 birds. Currently there are about
130 hatcheries (65 hatcheries with breeding farm) in Bangladesh, with
two million broilers and 0.3 million layer parent stock which producing
4 to 5 million commercial day old chicks per week. The commercial
broiler and layer farms supplying about 0.2 million metric ton of
poultry meat and 5210 million table eggs per year in Bangladesh.
(Samad, M.A. 2005). It fulfills one of the important sources of animal
protein. Diseases caused by interobacteria hamper the profitable broiler
production.
Keywords
ENTEROBACTERIA FROM APPARENTLY HEALTHY, Isolation and identification of Salmonella spp and E. coli, Preparation of Nutrient agar media