ISOLATION AND MOLECULAR CHARACTERIZATION OF BACTERIAL PATHOGENS FROM DIARRHOEAL CASES IN TIGER AND LION AT DHAKA ZOO

Abstract

The present study was conducted to isolate, identify and characterize the bacterial pathogens their antimicrobial resistance and molecular detection of E. coli and Salmonella of tiger and lion during the period from December 2013 to June 2014 in Dhaka Zoo. In this study there were 42 (twice from 6 tiger and 15 lion) faecal samples collected from the cages of animals just after defecation and brought to the Microbiology laboratory of the Department of Microbiology, Hajee Mohammad Danesh Science and Technology Universiy, Dinajpur for bacteriological examination. Isolation and identification of the microorganisms were confirmed by their morphology on different cultural media, staining characteristics and biochemical tests. Then, the isolated bacteria were characterized by antimicrobial susceptibility analyzed by the online software ABIS Encyclopedis/tgw1916, 2014 and detected by polymerase chain reaction (PCR) for E. coli primers (oligonucleotides) were FR ECO-1 & RV ECO-2 585 bp Pure Link™ Genomic DNA mini kit (Invitrogen, USA) and for Salmonella primers (oligonucleotides) were Fw & Rv 496 bp Wizard® Genomic DNA Purification Kit (promega, USA) were used for DNA extraction and 1.5% Agarose gel stained with ethidium bromide was used for gel documentation. A total of 42 faecal samples were examined for the isolation of bacteria of which 17 (40.47%) samples were positive for E. coli, 15 (35.71%) samples were positive for Salmonella sp, 18 (42.85%) samples were positive for Staphylococcus sp. The antibiogram study revealed that most of the E. coli, Salmonella spp. and Staphylococcus spp. were resistant to Doxycycline, Norfloxacin, Ciprofloxacin, Neomycin, Enrofloxacin, Ciprofloxacin, amoxicillin, Levofloxacin and Tetracycline. However, most of the E. coli, Salmonella sp. and Staphylococcus sp. were susceptible to Neomycin, Gentamicin and Colistine sulphate this is to indicate that the use of these antimicrobial may have the preference to be choice in clinical control of Salmonella, E. coli, and Staphylococcus sp infection. In molecular study, there were 11 isolates resulted as 7 (3 E. coli and 4 Salmonella) samples had positive in PCR both tiger and lion. Most of the isolates have zoonotic importance and confirmed the presence of either commensal or pathogenic for tiger and lion in zoo.