ISOLATION AND IDENTIFICATION OF SALMONELLA AND SHIGELLA SPECIES COLLECTED FROM HUMAN STOOL AT DINAJPUR GENERAL HOSPITAL, BANGLADESH

Abstract

Salmonellosis and Shigellosis bear the potential threat for human health. Outbreaks of Salmonellosis and Shigellosis remain serious health problems worldwide. Therefore, the present study was conducted to isolate and identify Salmonella and Shigella species from stool samples of human patients. A total of 33 samples were collected from the Dinajpur General Hospital in Bangladesh and brought to the laboratory of Microbiology, Faculty of Veterinary and animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur. This study was performed by morphological, cultural, biochemical test, serological test. In addition, antibiotic sensitivity was evaluated by disc diffusion method during the period from January 2020 to June 2020. The present study was obtained that the percentage of positive cases of Salmonella was 52.7% while the percentage of positive cases of Shigella was 28.6%. The study revealed that the prevalence of Salmonella and Shigella isolated from human stool samples related to male and female was (57.1% and 44.4%) respectively. The current study indicated that the male was higher prevalence than the female. Out of 17 patients 6 were positive and others were negative according to their age, the age of these participants were ranged 5- 15 years found (28.5%) (n=2) and (14.2%) (n=1) and 16-45 years of age obtained of result (30%) (n=3) and (0.0%) (n=0) was found positive in Salmonella and Shigella respectively. Also the overall prevalence related to age was (42.7% and 30%) of Salmonella and Shigella respectively. Salmonella species isolated, out of 33 patients were susceptible to Gentamicin, Cefixime and Chloramphenicol antibiotics. The remaining patients displayed resistant to Cefoxitin, Erythromycin, Azithromycin, Amoxicillin, Tetracycline and Ampicillin while intermediate to Levofloxacin. On the other hand, Shigella species were sensitive to Gentamicin, Azithromycin and Chloramphenicol. The isolates were resistant to Cefoxitin, Erythromycin, Levofloxacin, Amoxicillin, Tetracycline and Ampicillin while intermediate to Cefixime. The present study showed that the prevalence of Salmonella typhi, Salmonella paratyphi A and Salmonella paratyphi B using qualitative slide agglutination reaction results of Widal test was 39.4%, 12.1% and 6.1%, respectively.