IDENTIFICATION AND CHARACTERIZATION OF Escherichia coli FROM DRESSED CHICKEN MEAT IN DHAKA

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2019-12

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DEPARTMENT OF MICROBIOLOGY AND PARASITOLOGY, SHER-E-BANGLA AGRICULTURAL UNIVERSITY, DHAKA-1207

Abstract

Poultry is a major fast growing source of meat in the recent world. The consumers want a contamination free, safe and hygienic meat, which is free from any type of hazardous organism. This study has been done to find out the prevalence of Escherichia coli in different markets. The markets were different live bird markets and supermarkets. Also this study indicates the difference of Escherichia coli prevalence between live bird markets and the supermarkets in Dhaka city, Bangladesh. Samples from different markets were analyzed to determine the pathogenic bacteria Escherichia coli. It was isolated from the dressed meat sample of chicken through conventional cultural method. The samples were collected from twelve markets. Seven of the markets were live bird markets and other five were supermarkets. The markets were indicated as M1, M2, M3 etc for advantage while sampling. The markets were Savar Hut (M1), Shemulia Bazar (M2), Bagbari Bazar (M3), Bihari Camp (M4), Krishi Market (M5), Gigatola Bazar (M6), Taltola Bazar (M7), and Supermarkets were (M8), (M9), (M12), (M11) & (M10). From each market three samples were collected (S1, S2, S3). Result of this study demonstrated that the prevalence of E. coli was more in live bird market than the supermarkets. The average CFU/ml in live bird markets was 1.17534 × 10 12 and the average CFU/ml of supermarkets was 3.37327 × 10 11 . The E. coli prevalence percentage of live bird markets was 80.95% and in supermarkets it was 46.67%. So the pathogenic organism in the consumed meat of this type is alarming. This study also concluded recommendations that the personnel hygiene is crucial in processing and handling of poultry and poultry products.

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A Thesis Submitted to the Department Of Microbiology and Parasitology, Sher-e-Bangla Agricultural University, Dhaka-1207, in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (MS) IN MICROBIOLOGY DEPARTMENT OF MICROBIOLOGY AND PARASITOLOGY SEMESTER: JULY- DECEMBER, 2019

Keywords

IDENTIFICATION AND CHARACTERIZATION, Escherichia coli, DRESSED CHICKEN MEAT, DHAKA

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