QUALITATIVE AND QUANTITATIVE ASSESSMENT OF BOVINE OOCYTES

Abstract

The reproductive development of cattle is must to hasten the production of milk and meat. It relies mostly on the quality of oocytes. Thus the present study was carried out to sort out the superior ovary to be used in further research. Cow ovaries were collected from slaughter house, processed and categorized as right or left and ovaries with or without corpus luteum (CL). Morphology of ovaries were studied on the basis of length (cm), width (cm), weight (g), total number of follicles on the surface of each category ovaries, number of follicles aspirated, total number of cumulus-oocyte-complex (COCs) and then graded as normal and abnormal. Significantly (p<0.05) higher mean weight (3.47±0.34 vs 2.44±0.32), length (1.94±0.06 vs 1.71±0.07) and width (1.36±0.06 vs 1.09±0.05) were found in right ovaries than left. Forty seven per cent of right and 20% of left ovaries were found with CL. Numerically higher number of normal (0.97±0.16 vs 0.67±0.15) and total (1.60±0.18 vs 1.40±0.21) COCs was found in left ovaries than right ovaries whereas abnormal COCs were higher in right ovaries (0.73±0.14 vs 0.63±0.13). On the other hand, in comparison between with and without CL ovaries, significantly (p<0.05) higher mean weight (3.81±0.38 vs 2.53±0.29), length (2.04±0.07 vs 1.72±0.06) and width (1.45±0.06 vs 1.11±0.05) were found in with CL ovaries than without CL. The average number of normal COCs of with CL ovary (0.75±0.19) was lower than that of without CL (0.85±0.13) but they are not significant (p<0.05). Finally, it can be concluded that, oocytes from left ovaries in comparison to right and without CL in comparison to with CL are superior in the context of selection of oocytes for embryo development.