EFFECT OF PHYTOHORMONES IN OVERCOMING THE CROSS INCOMPATIBILITY BARRIER OF Corchorus capsularis AND Corchorus olitorius Hybrids

Abstract

This research work was carried out for producing inter-species putative hybrids of two cultivated jute species Corchorus capsularis and Corchorus olitorius by applying phytohormones and evaluating hybrid performance. This study was organized and executed in two seasons, first season for producing inter-species putative hybrids through applying phytohormones and the second season the performances the of the putative hybrids were evaluated. Five varieties of C. capsularis and six varieties of C. olitorius were used for interspecies hybridization programme. The eleven parents and their fourteen inter-species putative

hybrids were used for estimation of heterosis and character association study. Two hormone

treatments,

IAA and mixed hormones (GA 3 +NAA+Kinetin) were used to increase better embryo development and seed setting. Results showed that hormone treatments affected in fruit and seed setting. IAA gave better performance in seed setting and mixed hormones gave better performance in fruit setting. In case of the fruit setting, it was 41.21% in IAA and 46.0% in mixed hormones. While in case of seed setting, 13.80% in IAA and 7.33% in mixed hormones. The analysis of variance showed that there were significant variations among the performance of the parents and putative hybrids for the all the traits except base diameter and dry fiber weight. In case of hybrids, the higher heterosis value for plant height, base diameter, petiole length, root length, root weight, fresh weight with leaves, fresh weight without leaves, dry stick weight and dry fiber weight were 65.381% (OM-1 X CC-45), 36.065% (O-9897 X BJRI-2142), 34.444% (O-3820 X CVE-3), 48.995%( JRO-524 XCVL-1), 174.04% (O-9897 X BJRI-2142), 352.380% (O-72 X BJRI-2197), 347.27% (O-72 X BJRI-2197), 275.04%( O9897 X BJRI-2142) and 175.46% (JRO-524 XCVL-1) over better parent values respectively.

In

the correlation coefficient study, almost same result was observed in parents and interspecies putative hybrids at phenotypic levels. Correlation coefficient of the parents and interspecies hybrids clearly indicated that plant height, base diameter, petiole length, root length,

root weight,

fresh weight with leaves, fresh weight without leaves, dry stick weight and dry fiber weight, characters have very high degree of positive relationships in all cases. Taking together, IAA hormones had more effect in overcoming the cross incompatibility barrier between two cultivated jute species. Again, considering all characters the highest mean performance and heterosis value was in found in O-72 X BJRI-2197 cross combination indicating this combination would give transgressive segregants in later generations.