TRANSMISSION OF VIRUSES CAUSING MAIZE LETHAL NECROSIS DISEASE (MLND) THROUGH SEED IN COMMERCIAL HYBRIDS AND THEIR MOLECULAR DETECTION THROUGH RT- PCR

Abstract

ABSTRACT

An experiment was conducted in pot and laboratory condition under the Department of Plant Pathology of Sher-e-Bangla Agricultural University, Dhaka-1207 during 2020-2021.The pot experiment was carried out in a Completely Randomized Design (CRD) with ten replications. RT-PCR test was done in Molecular Biology and Plant Virology Laboratory. In this experiment ten varieties/lines viz., Tain 927, Jonaki 707, Rocket 55, Konok 51, Kohinoor, Line A.S, Line A, Line 984, Khoi Bhutta and Sweet corn were selected for conducting the experiment. Among the ten tested varieties/lines, Tain-927, Rocket-55 and Line A were showed tolerant. Variety Khoi bhutta was showed highly susceptible against Maize Lethal Necrosis Disease (MLND). Remaining varieties were showed susceptible to moderately susceptible. Molecular study was also performed for the detection of targeted maize viruses that cause MLN disease. From molecular study through RT-PCR test, it was revealed that results obtained on the basis of biological properties. All the selected varieties/lines gave the positive results in RT-PCR test against Maize Chlorotic Mottle Virus but showed negative result against Sugarcane Mosaic Virus. Different morpho-physiological, yield and yield contributing characters were also studied. Among the selected varieties, the maximum height of plant was obtained in the variety Tain-927 and minimum was obtained in the variety Khoi bhutta. The highest chlorophyll content was recorded in the variety Konok-51 and the lowest was recorded in the variety Kohinoor. The maximum number of fruits fruit weight and length was obtained from variety Tain-927 and the minimum was obtained from variety Khoi bhutta. Both disease incidence and severity show negative relation with plant height and chlorophyll content. From the study, it may be concluded that maize viruses were transmitted through hybrid seeds.