ISOLATION, IDENTIFICATION AND ANTIBIOGRAM STUDY OF BACTERIA FROM QUAIL FAECAL SAMPLES IN SYLHET SADAR

Abstract

The study was conducted to isolate and identify different bacteria from Quail in Sylhet Sadar. The study sample was faeces of Quail and was collected from Sarkar Quail and Poultry Hatchery, Kashem Quail and Breeder Hatchery and Shibganj Bazar, Sylhet. The samples were brought to the laboratory of Microbiology and Immunology Department, Faculty of Veterinary and Animal Science, Sylhet Agricultural University, Sylhet for isolation, identification and antibiogram study. About 90 samples were examined of which 30 samples from Sarkar Quail and Poultry Hatchery, 30 samples from Kashem Quail and Breeder Hatchery and 30 samples from Shibganj Bazar, Sylhet. Out of 30 samples from Sarkar Quail and Poultry Hatchery 23 samples were positive for Escherichia coli, 20 samples were positive for Salmonella spp. and 05 samples were positive for Pseudomonas spp. Out of 30 samples from Kashem Quail and Breeder Hatchery 25 samples were positive for Escherichia coli, 23 samples were positive for Salmonella spp. and 06 samples were positive for Pseudomonas spp. Out of 30 samples from Shibganj Bazar 26 samples were positive for Escherichia coli, 22 samples were positive for Salmonella spp. and 07 samples were positive for Pseudomonas spp. The percentage of positive samples from Sarkar Quail and Poultry Hatchery for Escherichia coli, Salmonella spp. and Pseudomonas spp. were 76.67%, 66.67% and 16.67% respectively. The percentage of positive samples from Kashem Quail and Breeder Hatchery for Escherichia coli, Salmonella spp. and Pseudomonas spp. were 83.33%, 76.67% and 20.00% respectively. The percentage of positive samples from Shibganj Bazar for Escherichia coli, Salmonella spp. and Pseudomonas spp. were 86.67%, 73.33% and 23.33% respectively. Antibiogram profiles indicate that E. coli isolated from apparently healthy quails were sensitive to Colistin sulphate, Neomycin, Ciprofloxacin, Chloramphenicol and totally resistant to Cloxacillin and Amoxacillin. Salmonella spp. were 100% sensitive to Ciprofloxacin, Colistin sulphate and 100% resistant to Amoxicillin, Ampicillin and cloxacillin. Pseudomonas spp. were fully sensitive to Ciprofloxacin and Colistin sulphate and totally resistant to Amoxicillin, Ampicillin and Cloxacillin.