BACTERIOLOGICAL ANALYSIS WITH ANTIBIOTIC RESISTANCE PATTERN OF BACTERIA ISOLATED FROM FRESH FRUIT JUICES OF DINAJPUR CITY, BANGLADESH

Abstract

Freshly prepared fruit juices sold by local market vendors in Dinajpur city were analyzed for the bacteriological quality. Forty (40) fresh fruit juices were collected from different areas around Dinajpur city. Standard plate count techniques were followed to assess Total viable bacterial count (TVC), Total coliform count (TCC), and Total Staphylococcal count (TSC). The total viable bacterial count ranged from 1.8×104 to 5.6×107cfu/ml. Total coliform count and Total Staphylococcal count of juice samples were ranged from 1.36×103 to 5.76×106 cfu/ml and 0 to 5.85×106 cfu/ml respectively. The juice samples were also found to be contaminated with Escherichia coli, Staphylococcus spp., Salmonella spp. & Klebsiella spp. Out of 40 freshly prepared fruit juice samples collected, 38 samples (95%) showed the presence of E. coli. The percentage of Staphylococcus spp., Salmonella spp. & Klebsiella spp. of the tested samples were 75%, 12.5%, and 10% respectively. So the fruit juice samples were unsafe for drinking. Antibiotic resistance pattern of the isolated bacteria revealed that, among the four isolated bacteria E. coli, Salmonella spp., Klebsiella spp. were 100% resistant to Ampicillin but Staphylococcus spp. were 87% resistant to Ampicillin. Isolated Klebsiella spp. was found to be 75% resistant to Amoxicillin and Salmonella spp. were 20% resistant to Vancomycin. Such drug resistance properties may render these pathogens cause serious health hazards because of ineffective treatment of the sufferers by the commonly prescribed antibiotics. It was concluded that due to unhygienic fruit handling in the unsanitary environmental conditions vi the juices become contaminated with bacteria which are harmful for public health. Key words: Fruit juice, TVC, TCC, TSC, Antibiotic Resistance pattern.