DETECTION OF MICROBES WITH THEIR CHARACTERIZATION CAUSING STUNTED GROWTH IN COMMERCIAL BROILERS
Date
2011-08
Authors
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Publisher
HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR.
Abstract
The study was conducted to isolate and characterize the microbes causing stunted growth in
commercial broilers from Vai-Vai Poultry Farm (Kornai, Katapara, Dinajpur), Israfil
Poultry Farm (Basherhat, Dinajpur), Guljar Poultry Farm (North Sibrampur, Dinajpur)
and Maa Poultry Farm (Nayanpur, Dinajpur), during the period from January 2011 to July
2011. A total of 158 samples comprising dead birds, sick birds, litter, droppings, poultry
feed and drinking water were collected among them 56 (n= 56) positive samples were
isolated for this study from commercial broilers and subjected to primary isolation by
propagating in nutrient broth followed by culture on selective media— Brilliant Green Agar,
Salmonella-Shigella Agar, Eosin Methylene Blue Agar and Sabouraud’s Dextrose Agar
media. Gram's staining techniques were performed. Biochemical properties of the isolates
were studied and reaction in TSI agar slant was also observed. Among the 56 positive
isolates 9 isolates were found positive for Fungi, 37 isolates were found positive for E. coli
and 26 isolates were found to be positive for Salmonella spp. that are the casual factors for
stunted growth in commercial broilers. The prevalence of Fungi, E. coli and Salmonella
spp. were recorded as 16.07%, 66.07% and 46.42% respectively. Among the microbes
isolated Escherichia coli was determined as predominant bacteria (66.07%) causing stunted
growth in commercial broilers than Salmonella spp. (46.42%) and Fungi (16.07%). Litter
and dropping samples were the highest sources of contamination than tracheal swabs.
Fungal samples were isolated from feed, litter and drinking water samples and the
prevalence of Fungi were recorded as lowest (16.07%) than other microbes causing stunted
growth than Escherichia coli (66.07%) and Salmonella spp. (46.42%).
Description
Bangladesh is an agricultural country with a large number of domestic
chickens and ducks. About 80% of the total population of 140 million is
living in the 68,000 villages of Bangladesh, and almost each and every
village home holds 6-7 chickens. It is estimated that there are about 153
million chickens in Bangladesh. There are about one lac poultry farms in
Bangladesh, of which 20% rearing 1000 to 50,000 birds and remaining
80% are small in size with 100 to 1000 birds. Currently there are about
130 hatcheries (65 hatcheries with breeding farm) in Bangladesh, with
two million broilers and 0.3 million layer parent stock which producing 4
to 5 million commercial day-old-chicks per week. The commercial broiler
and layer farms supplying about 0.2 million metric ton of poultry meat
and 5210 million table eggs per year in Bangladesh. (Samad, M.A. 2005).
Keywords
Body weight measurement and record keeping, Standard Body weight of Broiler, Preparation of Nutrient broth