Phytochemical Screening and Dpph Free Radical Scavenging Activity of Centella Asiatica (L)

Abstract

The present study was aimed at investigating the chemical profiles of the methanolic extract of leaves of Centella asiatica as well as to assess its antioxidative activity. In this study, a methanolic extract (50 g/L) was obtained by infusion followed by cold maceration for 24 h. The phytochemical screening of the plant extract demonstrated the presence of several classes of compounds like alkaloids, flavonoids, tannins and steroids. The concentration of the plant extract needed for 50% scavenging (IC50) of DPPH was found to be 40.4 μg/ml whereas IC50values for the positive control was found to be 16.34 μg/ml for BHT and 65.03 μg/ml for Ascorbic acid (AS).