CHARACTERIZATION AND ANTIBIOGRAM STUDY OF BACTERIAL PATHOGENS ISOLATED FROM OMPHALITIS IN CALVES
Date
2012-12
Authors
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Publisher
HAJEE MOHAMMOD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR
Abstract
The study was conducted for characterization and antibiogram study of bacterial
pathogens isolated from Omphalitis in calves. The Study was performed by
using staining, cultural, biochemical test and antibiogram study of identified
filled isolates during the period of July’2012 to December’2012. A total of
21 pus samples were collected from Omphalitis affected calves. All the samples
were collected from the different veterinary hospital under Rangpur district for
this study. The positive samples were brought to the Microbiology laboratory
of the Department of Microbiology, Faculty of Veterinary and animal Science,
Hajee Mohammad Danesh Science and Technology University, Dinajpur. A
total of 21 pus samples from infected calves belonging to group-A (13), B (6)
and C (2) and comprising 13, 6, 2 sample respectively were inoculated into
different bacteriological media and stained by Grams staining technique. It was
observed that the highest percentage of occurrence of Omphalitis obtained from
group-A: (61.90%) followed by group-B: (28.55%) and group-C: (9.52%). It
was observed that out of 21 isolates Staphylococcus species was 66.67% and E.
coli was 33.33%. Overall sensitivity pattern of antibiotics to Omphalitis
revealed that Levofloxacin, Penicillin, Ciprofloxacin and Azithromycin are
most efficacious followed by Chloramphenicol and Doxycycline. Thus, it may
be recommended that Ciprofloxacin, Penicillin and Levofloxacin in optimum
doses would resolve most cases of Omphalitis in calves
Description
Newborn calves are vulnerable to many types of infection and disease.
Navel ill, or “Omphalitis,” is a common problem when calves are born in a
dirty environment, such as a pen or pasture where cattle have congregated,
or a barn stall that hasn’t been cleaned between calvings. Wherever there is
manure, discharges from cows that have calved, or cattle with foot rot or
other infections, there is risk of infection to newborn calves (Heather Smith
Thomas, 2003).
Keywords
Collection of Sample, Transportation and Preservation of Samples, Laboratory Preparation, Media for culture