In Vitro Sensitivity Study of Different Brands of Ornidazole against Clinical Isolates of Entamoeba histolytica

Abstract

Entamoeba histolytica is the etiological agent of amoebic dysentery and amoebic liver abscess. Amoebiasis is one of the most common health problems in the developing countries. Almost 10% people of the world are suffering from amoebiasis. Poverty, ignorance, overcrowding, poor sanitation and malnutrition favor transmission and increased disease burden. The main objective of the study was to find out the in vitro sensitivity of different brands of Omidazole against clinical isolates of Entamoeba histolytica. Entamoeba histolytica isolates was collected from the stool sample and cultured in different culture medium. Different brands of Omidazole were prepared in different concentrations of 4.6, 2.3 ,1.15 , 0.575 , 0.287 and 0.143 JlM using the Di-methyl sulphoxide (DMSO) solvent and incubated at 24 hours respectively. After incubation the isolates of viable Entamoeba histolytica were calculated. Viable count of the Entamoeba histolytica in each concentration of Omidazole (brands) were compared to the initial concentration and with the Omidazole standard. After 24 hours of incubation concentration of 4.6 JlM showed very few amounts of Entamoeba histoiytica. As the concentrations of Omidazole (brands and standard) decreased (4.6, 2.3 ,1.15 , 0.575 , 0.287 and 0.143 J!M), after 48 hours of incubation the viability of clinical isolates of Entamoeba histolytica increased These results showed that Omidazole brands were effective to inhibit the growth of clinical isolates Entamoeba histolytica.