ISOLATION AND IDENTIFICATION OF PASTEURELLA MULTOCIDA FROM DUCKS AT DINAJPUR DISTRICT OF BANGLADESH AND THEIR ANTIBIOGRAM STUDY

Abstract

The present research work was performed for the isolation and identification of Pasteurella multocida from ducks and demonstration of antibiotic sensitivity of the selected isolates. The entire research work was conducted primarily in the Bacteriology laboratory of the Department of Microbiology, Hajee Mohammad Danesh University (HSTU), Dinajpur. The samples were aseptically carried out to the laboratory and were subjected to different cultural, morphological and biochemical examinations. For this purpose, a total of 20 samples (liver, spleen, heart) were collected from suspected sick ducks (10 samples) and suspected dead ducks (10 samples) from different selected areas of Dinajpur district. From these samples, 3 (30 %) from suspected sick ducks and 4 (40 %) from suspected dead ducks were found to be positive for Pasteurella multocida. The isolated organism from ducks produced whitish, opaque, round, flat, translucent colonies of sticky mucoid or dry consistency about 1-3 mm in diameter on Nutrient Agar and whitish, opaque, round, translucent colonies without hemolysis on Blood Agar and were able to ferment dextrose, mannitol and sucrose but unable to ferment lactose and maltose on biochemical examinations. From the antibiogram study, it was revealed that among the isolated Pateurella organism from ducks, 100% were highly sensitive to Ciprofloxacin and 86% Levofloxacillin, 71% were to Clindamycin and 14% were to Gentamycin. 71% were moderately sensitive to Doxycicline, 86% were to Gentamycin, 15% were to Clindamycin, 15% were to Neomycin and 33% were to Erythromycin. On the other hand 57% were less sensitive to Erythromycin; 17% were less sensitive to Tetracycline; 14% were to Doxycicline and 8% were to Bacitracin. 100% isolates were resistant to Amoxycillin, where 83% were resistant to Tetracycline; 92% were resistant to Bacitracin, and 20% were resistant to Erythromycin.