Pharmacological Investigation of Leaves of Streblus asper

Abstract

The study was designed for pharmacological investigation of different extract of leaves of Streblus asper (Family: Moracea, Bengali name – Shaora (শেওড়া) and screening of their biological activities like, antimicrobial, cytotoxicity and free radical scavenging activities. The powdered leaves of Streblus asper was extracted with methanol. The concentrated extract was then partitioned with N-hexane, Dichloromethane, and Ethylacetate. In antimicrobial activity investigation, methanolic crude extract, dichloromethane fraction, ethylacetade fraction & n-hexane fraction of methanolic extract of Streblus asper (leaves) showed moderate activity against the tested organisms. They were appeared almost no zone of inhibition against gram positive & gram negative bacteria. The range of zone of inhibition was 8.5-10mm, 8-9.25mm, 6.00-7.50mm,7.0-8.0mm respectively dichloromethane, ethylacetade, nhexane and methanolic extract. Which is compared to kanamycin (30 μg/disc) used as positive control in this study. Bioactivity guided isolation can be carried out to separate bioactive metabolites from these extracts. Methanolic crude extract of Streblus asper (leaves) and its different fractions were screened for cytotoxic properties using brine shrimp lethality bioassay where Vincristine sulfate (LC50 value 3.23 μg/ml) was used as a positive control. From the results of the brine shrimp lethality bioassay, it can be predicted that the methanolic crude extract, dichloromethane fraction, ethylacetade fraction & n-hexane fraction of methanolic extract possess cytotoxic principles and have cytotoxic potency having LC50 values of 0.025 μg/ml, 3.67μg/ml, 21.96 μg/ml and 0.29μg/ml respectively. The degree of lethality of brine shrimp increases in a dose dependent manner. b In evacuation of total phenolic content of different extraction of Streblus asper. The total phenolic content of methanolic crude extract, dichloromethane fraction, ethylacetade fraction & n-hexane fraction of methanolic extract is respectively is 131.70mgGAE/g, 122.50mgGAE/g, 154.81mgGAE/g, 96.79mgGAE/g. In evaluation of free radical scavenging activity it was found that crude methanolic extract of Streblus asper (leaves) exhibited remarkable antioxidant activity with much lower IC50 value. IC50 value of methanolic crude extract, dichloromethane fraction, ethylacetade fraction & nhexane fraction of methanolic extract is respectively is 0.696 μg/ml, 0.427 μg/ml, 0.414μg/ml, 0576μg/ml.