BIOCHEMICAL INVESTIGATION OF RADISH ROOT TOWARDS THE DEVELOPMENT OF BOTANICAL FUNGICIDE AGAINST Alternaria
Date
2010-08
Authors
Journal Title
Journal ISSN
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Publisher
HAJEE MOHAMMOD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR
Abstract
The root of Tasaki Sun Mula (Bari Mula-1) was used to determine the antifungal
biochemical substances and the effectiveness of its aqueous extract on the growth
of Alternaria brassicae. Different solvents like polar nature viz. water, methanol,
ethanol and as well as non-polar nature viz. Petroleum ether, Chloroform were used
for extraction. Among the solvents, methanol extract contained the highest amount
of crude products (15.2g) whereas ethanol extract (9.95g), water extract (8.94g),
chloroform extract (0.91g) and petroleum ether extract contained (0.63g). During
TLC, ethanol crude extract produced four distinct spots at hexane and ethyl acetate
(20:1; v/v). The different compounds detected by TLC were designated as R;- Rg
where R;, is the most non-polar and Rg is the most polar compound where the
polarity of different compounds may be arranged as R;<R2<R3<R4<R5<Rg, The
TLC of aqueous extracts were not possible due to solubility. From the nature of
TLC it is very clear that R; is probably waxy type hydrocarbon functioning as
mimic in plant defense. In the study of aqueous extract, three different types of
media viz. PDA, Radish Leaf Extract Media (RLEM) and Radish Root Extract
Media (RREM) were prepared for observing the growth of Alternaria brassicae.
Up to seven days, the mycelia growth on both selective media were same (7 cm)
but after seven days mycelial growth was continued on RLEM and turned into
blackish color whereas mycelia growth was stunted on RREM and remained
whitish color. Abundant numbers of conidia were found in RLEM but no conidium
was found in RREM. There was no growth in PDA media. Finally it might be
concluded that Radish root may contain six different biochemical compounds
functioning as anti pathogenic to Alternaria brassicae.
Description
Radish (Raphanus sativus, 2n=18) under the family Brassicaceae is the third most
important vegetable crop in Bangladesh (Rashid, 2009). Radishes are rich
in ascorbic acid, folic acid, and potassium. They are a good source of vitamin
Bg, riboflavin, magnesium, copper, and calcium. One cup of sliced red radish bulbs
provides approximately 20 calories, largely from carbohydrates. Radish root has
some anti pathogenic properties (Rashid, 2005). Radish seeds have previously been
shown to contain two homologous, 5-kD cysteine-rich proteins designated
Raphanus sativus-antifungal protein 1 (Rs-AFP1) and Rs-AFP2, both of which
exhibit potential antifungal activity in vitro (Terras et al. 1993).
Keywords
Experimental Site:, Solvent were used:, Biochemical Investigation of Radish Root, Plant materials collection, Plant Pathology