ISOLATION, IDENTIFICATION AND ANTIBIOGRAM STUDY OF PASTEURELLA MULTOCIDA FROM CHICKENS AT DINAJPUR DISTRICT OF BANGLADESH
Date
2010-08
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR.
Abstract
The present research work was performed for the isolation and characterization of
Pasteurella multocida from recent outbreak of chickens and demonstration of
antibiotic sensitivity of the selected isolates. The entire research work was conducted
primarily in the Bacteriology laboratory of the Department of Microbiology, Hajee
Mohammad Danesh Science and Technology University (HSTU), Dinajpur. The
samples were aseptically carried out to the laboratory and were subjected to different
cultural, morphological and biochemical examinations.
A number total of 106 samples inluding liver, spleen and heart were collected from
suspected sick chickens (60 samples) and suspected dead chickens (46 samples) from
different selected areas of Dinajpur district. From these samples, 25 (42 %) from
suspected sick chickens and 11 (24 %) from suspected dead chickens were found to be
positive for Pasteurella multocida.
The isolated organism from chickens produced whitish, opaque, round, flat, translucent
colonies of sticky mucoid or dry consistency about 1-3 mm in diameter on Nutrient
Agar and whitish, opaque, round, translucent colonies without hemolysis on Blood
Agar and were able to ferment dextrose, mannitol and sucrose but unable to ferment
lactose and maltose on biochemical examinations.
Form the antibiogram study, it was revealed that among the isolated Pateurella
multocida from chicken, 100% were highly sensitive to Ciprofloxacin and
Levofloxacin. 83% were to Neomycin, 78% were to Clindamycin and 3% were to
Gentamycin. 75% were moderately sensitive to Doxycicline, 61% were to
Gentamycin, 22% were to Clindamycin, 17% were to Neomycin and 14% were to
Erythromycin. On the other hand 61% were less sensitive to Erythromycin; 17% were
to Tetracycline; 13% were to Gentamicin, 25% were to Doxycicline and 8% were to
Bacitracin. 100% isolates were resistant to Amoxycillin, 83% were to Tetracycline;
92% were to Bacitracin, and 25% were to Erythromycin.
Description
Poultry industry in Bangladesh is growing day by day. It is an excellent agribusiness,
started practically during 1980s in Bangladesh (Haque, 2001). There has been a
tremendous development of this sector since last decades (1996-2006) in the country
(Rahman, 2003). The sector has already proved itself as a potential income generator and
poverty alleviator. At present, there are more then 135 hatcheries producing millions of
day old-chicks per week continuously and about one million commercial broiler and layer
farms supplying million kgs of meat and about 12 million table eggs per week. But it is
true to say that there are factors which are hindering expansion of poultry industries in
Bangladesh. Of these, infectious diseases are considered as the most leading causes of
economic loss and discouraging poultry rearing in this country (Das et al., 2005). Among
the infectious diseases, fowl cholera is a major threat to the poultry industry.
Keywords
Growth of Pasteurella multocida in Nutrient Broth indicated by the presence of diffused turbidity., Growth of Pasteurella multocida on EMB agar media showing small, circular, convex, translucent, glistening colonies., Microscopic photograph of Pasteurella multocida in Methylene blue staining.