Browsing by Author "Tamanna, Sonia"

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Aptamer biosensor design for the detection of endocrine-disrupting chemicals small organic molecules using novel bioinformatics methods
(Scopus, 2024-09-30) Bayıl, Imren; Hossain, Md. Sarowar; Tamanna, Sonia; Uddin, Md Jamir; Ahamed, .M. Mashood; Jardan, Yousef A. Bin; Bourhia, Mohammed; Tok, Tugba Taskin
Endocrine-disrupting chemicals (EDCs) are substances that can disrupt the normal functioning of hormones . Using aptamers, which are biological recognition elements, biosensors can quickly and accurately detect EDCs in environmental samples. However, the elucidation of aptamer structures by conventional methods is highly challenging due to their complexity. This has led to the development of three-dimensional aptamer structures based on different models and techniques. To do this, we developed a way to predict the 3D structures of the SS DNA needed for this sequence by starting with an aptamer sequence that has biosensor properties specific to bisphenol-A (BPA), one of the chemicals found in water samples that can interfere with hormones. In addition, we will elucidate the intermolecular mechanisms and binding affinity between aptamers and endocrine disruptors using bioinformatics techniques such as molecular docking, molecular dynamics simulation, and binding energies. The outcomes of our study are to compare modeling programs and force fields to see how reliable they are and how well they agree with results found in the existing literature, to understand the intermolecular mechanisms and affinity of aptamer-based biosensors, and to find a new way to make aptamers that takes less time and costs less.
Aptamer Biosensor Design for the Detection of Endocrine-disrupting Chemicals Small Organic Molecules Using Novel Bioinformatics Methods
(Elsevier, 2024-09-15) Bayıl, Imren; Hossain, Md. Sarowar; Hossain, Md. Sarowar; Tamanna, Sonia; Uddin, Md Jamir; Ahamed, F.M. Mashood; Jardan, Yousef A. Bin; Bourhia, Mohammed; Tok, Tugba Taskin
Endocrine-disrupting chemicals (EDCs) are substances that can disrupt the normal functioning of hormones.Using aptamers, which are biological recognition elements, biosensors can quickly and accurately detect EDCs in environmental samples. However, the elucidation of aptamer structures by conventional methods is highly challenging due to their complexity. This has led to the development of three-dimensional aptamer structures based on different models and techniques. To do this, we developed a way to predict the 3D structures of the SS DNA needed for this sequence by starting with an aptamer sequence that has biosensor properties specific to bisphenol-A (BPA), one of the chemicals found in water samples that can interfere with hormones. In addition, we will elucidate the intermolecular mechanisms and binding affinity between aptamers and endocrine disruptors using bioinformatics techniques such as molecular docking, molecular dynamics simulation, and binding energies. The outcomes of our study are to compare modeling programs and force fields to see how reliable they are and how well they agree with results found in the existing literature, to understand the intermolecular mechanisms and affinity of aptamer-based biosensors, and to find a new way to make aptamers that takes less time and costs less.
Investigation of Analgesic, Anti-Inflammatory and Antidiabetic Effects of Phyllanthus Beillei Leaves H
(Scopus, 2021) Hossain, Md. Sarowar; Islam, M. Anwarul; Rahman, Md. Anisur; Tamanna, Sonia
The current study was carried out to explore the analgesic, anti-inflammatory and anti-diabetic potential of methanol extract of Phyllanthus beilei H. The analgesic activities were determined by acetic acid induced writhing test in mice. The oral administration of doses 500 mg/kg of methanol extract of Phyllanthus beilei H. inhibited 42.22% of writhing movements compared to standard drug diclofenac-Na 94.28% writhing movement inhibition induced by acetic acid. Anti-inflammatory effect of ethyl acetate extract was determined after oral administration of 1% Carrageenan, paw edema in mice. Mice treated with ethyl acetate extract of Phyllanthus beilei H. (500 mg/kg) moderately decreased in paw volume at 180 min and 240 min compared with mice of control group. Here, pronounced effects were observed in extract group 34.36% inhibition of paw edema compared to that of Standard Group 55.13% inhibition of paw edema. In the Oral glucose tolerance test the control group of mice exhibited higher blood glucose levels whereas, mice treated with extract (500 mg/kg) showed significant decrease in blood glucose level after 30 mins and 120 mins compared with control group of mice.
Phytochemical, Anti-Microbial and Cytotoxic Studies on Leaves Extract of Phyllanthus Beillei H
(Scopus, 2021) Hossain, Md. Sarowar; Islam, M. Anwarul; ghosh, Mrinal Kanti; Rashid, Md. Harun-Or; Tamanna, Sonia
Microbial resistant is getting dangerous day by day. To find a new antimicrobial source is very essential. The current research study was designed to assess the antimicrobial activity of methanol extract of Phyllanthus Billie H. by disc diffusion method. By measuring zone of inhibition mild antimicrobial activity of Bacillus subtilis was found and compared with the standard Ciprofloxacin. From the result, it can be concluded that the Phyllanthus beillei H. plant may be a source of antimicrobial constituents. The plant extract also demonstrated promising cytotoxic activity against brine shrimps nauplii. The value of LC50 of methanol extract of Phyllanthus Billie H. was 2.4119 mg/mL which compared with the LC50 value of the standard (0.0171mg/mL). The overall results indicate a strong cytotoxic activity of this plant.
Vitamin D deficiency and the vitamin D receptor (VDR) gene polymorphism rs2228570 (FokI) are associated with an increased susceptibility to hypertension among the Bangladeshi population
(Scopus, 2024) Mahmud, Zimam; Nabil, Imrul Kayes; Tamanna, Sonia; Rahman, Md Mostafijur; Rashid, Gowhar; Hossain, Md Sarowar; Asad, Humaira Binte; Kamal, Mohammad Amjad; Howlader, Md Zakir Hossain
Vitamin D receptor (VDR) gene is implicated in hypertension vulnerability due to its role in regulating the renin-angiotensin system (RAS) and blood pressure. In this case-control study, a carefully selected cohort of 111 hypertensive individuals and 100 healthy controls underwent serum analysis using HPLC to measure 25-hydroxy vitamin D levels. Polymorphic variations in the VDR gene were detected and characterized using the PCR-RFLP method. At first, lower 25-hydroxy vitamin D levels were observed in hypertensive individuals compared to controls (p<0.001). The genotype frequency of the VDR gene TaqI showed no significant difference between cases and controls (p>0.05). Similarly, no significant difference was found in the VDR gene BsmI genotype frequency between hypertensive patients and controls (p>0.05). However, a statistically significant distinction was observed in the VDR gene FokI genotype frequency between cases and controls (p<0.01). The odds ratios for FokI genotypes (CC, CT, TT, and CT+TT) were 1.0, 0.590, 1.566, and 0.963, respectively. Furthermore, serum 25-hydroxy vitamin D levels were significantly higher in control subjects compared to hypertensive patients across all genotypes of VDR (p<0.001). Hypertensive patients, excluding those with the FokI VDR gene CC genotype, exhibited significantly higher systolic blood pressure levels compared to the control group (p<0.05). Similarly, hypertensive subjects displayed elevated diastolic blood pressure levels compared to the control group (p<0.001). Overall, the results suggest the presence of a potential inverse correlation between serum 25-hydroxy vitamin D levels and hypertension. The association analysis conducted indicated that there is no significant association between TaqI and bsmI genotypic variants and the risk of developing hypertension. However, it was observed that VDR gene polymorphisms do have a clear association with hypertension susceptibility, as evidenced by the significantly higher occurrence of FokI genotypic variants in hypertensive patients. Our study therefore introduces the possibility of utilizing 25-hydroxy vitamin D deficiency and VDR gene polymorphisms as a biomarker for hypertension.
Vitamin D Deficiency and the Vitamin D Receptor (VDR) Gene Polymorphism rs2228570 (FokI) Are Associated With an Increased Susceptibility to Hypertension Among the Bangladeshi Population
(PLOS ONE Publications, 2024-03-14) Nabil, Imrul Kayes; Mahmud, Zimam; Tamanna, Sonia; Rahman, Md Mostafijur; Rashid, Gowhar; Hossain, Md. Sarowar; Asad, Humaira Binte; Howlader, Md. Zakir Hossain
Vitamin D receptor (VDR) gene is implicated in hypertension vulnerability due to its role in regulating the renin-angiotensin system (RAS) and blood pressure. In this case-control study, a carefully selected cohort of 111 hypertensive individuals and 100 healthy controls underwent serum analysis using HPLC to measure 25-hydroxy vitamin D levels. Polymorphic variations in the VDR gene were detected and characterized using the PCR-RFLP method. At first, lower 25-hydroxy vitamin D levels were observed in hypertensive individuals compared to controls (p<0.001). The genotype frequency of the VDR gene TaqI showed no significant difference between cases and controls (p>0.05). Similarly, no significant difference was found in the VDR gene BsmI genotype frequency between hypertensive patients and controls (p>0.05). However, a statistically significant distinction was observed in the VDR gene FokI genotype frequency between cases and controls (p<0.01). The odds ratios for FokI genotypes (CC, CT, TT, and CT+TT) were 1.0, 0.590, 1.566, and 0.963, respectively. Furthermore, serum 25-hydroxy vitamin D levels were significantly higher in control subjects compared to hypertensive patients across all genotypes of VDR (p<0.001). Hypertensive patients, excluding those with the FokI VDR gene CC genotype, exhibited significantly higher systolic blood pressure levels compared to the control group (p<0.05). Similarly, hypertensive subjects displayed elevated diastolic blood pressure levels compared to the control group (p<0.001). Overall, the results suggest the presence of a potential inverse correlation between serum 25-hydroxy vitamin D levels and hypertension. The association analysis conducted indicated that there is no significant association between TaqI and bsmI genotypic variants and the risk of developing hypertension. However, it was observed that VDR gene polymorphisms do have a clear association with hypertension susceptibility, as evidenced by the significantly higher occurrence of FokI genotypic variants in hypertensive patients. Our study therefore introduces the possibility of utilizing 25-hydroxy vitamin D deficiency and VDR gene polymorphisms as a biomarker for hypertension.