Browsing by Author "Haque, M. Ekramul"

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Antioxidant, analgesic and toxic potentiality of stephania japonica (Thunb.) miers. leaf
(© 2011 Asian Network for Scientific Information., 2011) Rahman, Md Habibur; Alam, Md. Badrul; Chowdhury, N.S.; Kumar Jha, Mithilesh; M, Hasan; Khan, Md. Maruf; Rahman, Md. Saifur; Haque, M. Ekramul
In the present study crude methanolic extract of Stephania japonica leaf was investigated for possible antioxidant, analgesic and toxic activity. The extract showed antioxidant activity in DPPH radical scavenging activity, nitric oxide scavenging activity and reducing power assays. In both DPPH radical and NO scavenging assay, the extract exhibited moderate antioxidant activity and the IC50 values in DPPH radical scavenging and NO scavenging assays were found to be 105.55±1.06 and 129.12±0.15 ug mL-1, respectively while the IC50 values of ascorbic acid were 12.30±0.11 and 18.64±0.22 ug mL-1, respectively. Reducing power activity of the extract increased in a dose dependent manner. Analgesic activity of the crude extract was evaluated using acetic acid-induced writhing model of pain in mice. The crude extract at 200 and 400 mg kg-1 b. wt. doses displayed significant (p<0.001) reduction in acetic acid induced writhing in mice with a maximum effect of 75.89% reduction at 400 mg kg-1 b.wt. which is comparable to the standard, diclofenac sodium (86.52%). The extract was also investigated for toxic potentiality using Brine Shrimp lethality bioassay. In this bioassay the extract showed significant toxicity to Brine Shrimp nauplii with the LC50 value of 25.19±0.98 ug mL-1. The study clearly indicates that the extract possesses good analgesic and cytotoxic activity along with moderate antioxidant potential.
Antioxidant, analgesic and toxic potentiality of stephania japonica (Thunb.) miers. leaf
(© 2011 Asian Network for Scientific Information., 2011) Rahman, Md Habibur; Alam, Md. Badrul; Chowdhury, N.S.; Kumar Jha, Mithilesh; M, Hasan; Khan, Md. Maruf; Rahman, Md. Saifur; Haque, M. Ekramul
In the present study crude methanolic extract of Stephania japonica leaf was investigated for possible antioxidant, analgesic and toxic activity. The extract showed antioxidant activity in DPPH radical scavenging activity, nitric oxide scavenging activity and reducing power assays. In both DPPH radical and NO scavenging assay, the extract exhibited moderate antioxidant activity and the IC50 values in DPPH radical scavenging and NO scavenging assays were found to be 105.55±1.06 and 129.12±0.15 ug mL-1, respectively while the IC50 values of ascorbic acid were 12.30±0.11 and 18.64±0.22 ug mL-1, respectively. Reducing power activity of the extract increased in a dose dependent manner. Analgesic activity of the crude extract was evaluated using acetic acid-induced writhing model of pain in mice. The crude extract at 200 and 400 mg kg-1 b. wt. doses displayed significant (p<0.001) reduction in acetic acid induced writhing in mice with a maximum effect of 75.89% reduction at 400 mg kg-1 b.wt. which is comparable to the standard, diclofenac sodium (86.52%). The extract was also investigated for toxic potentiality using Brine Shrimp lethality bioassay. In this bioassay the extract showed significant toxicity to Brine Shrimp nauplii with the LC50 value of 25.19±0.98 ug mL-1. The study clearly indicates that the extract possesses good analgesic and cytotoxic activity along with moderate antioxidant potential.
Antioxidant, anti-inflannnatory and anti-pyretic activities of trichosanthes dioica roxb. fruits
(© 2011 Academic Journals Inc., 2011) Alam, M. Badrul; Hossain, M. Sarowar; Chowdhury, N. Sultana; Asadujjaman, M.; Zahan, Ronok; Islam, M. Monirul; Mazumder, M. Ehsanul Haque; Haque, M. Ekramul; Islam, Anwarul
The present study was designed to investigate the antioxidant, anti-inflammatory and antipyretic potential of the methanolic extract along with its organic soluble fractions of the fruits of Trichosanthes dioica Roxb. Antioxidant potential of the extract/fractions was evaluated by DPPH (1,ldiphenyl-2-picrylhydrazyl) and NO (nitric oxide) scavenging assay method. Ethyl acetate fractions (EtOAc) showed highest scavenging activity in all the methods with IC50 value of 12.32±0.16 and 5.38±0.07 pg mL -1 for DPPH and NO assay method, respectively. In reducing power assay, EtOAc fraction also showed significant (p
Antioxidant, anti-inflannnatory and anti-pyretic activities of trichosanthes dioica roxb. fruits
(© 2011 Academic Journals Inc., 2011) Alam, M. Badrul; Hossain, M. Sarowar; Chowdhury, N. Sultana; Asadujjaman, M.; Zahan, Ronok; Islam, M. Monirul; Mazumder, M. Ehsanul Haque; Haque, M. Ekramul; Islam, Anwarul
The present study was designed to investigate the antioxidant, anti-inflammatory and antipyretic potential of the methanolic extract along with its organic soluble fractions of the fruits of Trichosanthes dioica Roxb. Antioxidant potential of the extract/fractions was evaluated by DPPH (1,ldiphenyl-2-picrylhydrazyl) and NO (nitric oxide) scavenging assay method. Ethyl acetate fractions (EtOAc) showed highest scavenging activity in all the methods with IC50 value of 12.32±0.16 and 5.38±0.07 pg mL -1 for DPPH and NO assay method, respectively. In reducing power assay, EtOAc fraction also showed significant (p
Antioxidant, anti-inflannnatory and anti-pyretic activities of trichosanthes dioica roxb. fruits
(© 2011 Academic Journals Inc., 2011) Alam, M. Badrul; Hossain, M. Sarowar; Chowdhury, N. Sultana; Asadujjaman, M.; Zahan, Ronok; Islam, M. Monirul; Mazumder, M. Ehsanul Haque; Haque, M. Ekramul; Islam, Anwarul
The present study was designed to investigate the antioxidant, anti-inflammatory and antipyretic potential of the methanolic extract along with its organic soluble fractions of the fruits of Trichosanthes dioica Roxb. Antioxidant potential of the extract/fractions was evaluated by DPPH (1,ldiphenyl-2-picrylhydrazyl) and NO (nitric oxide) scavenging assay method. Ethyl acetate fractions (EtOAc) showed highest scavenging activity in all the methods with IC50 value of 12.32±0.16 and 5.38±0.07 pg mL -1 for DPPH and NO assay method, respectively. In reducing power assay, EtOAc fraction also showed significant (p
Antioxidant, anti-inflannnatory and anti-pyretic activities of trichosanthes dioica roxb. fruits
(© 2011 Academic Journals Inc., 2011) Alam, M. Badrul; Hossain, M. Sarowar; Chowdhury, N. Sultana; Asadujjaman, M.; Zahan, Ronok; Islam, M. Monirul; Mazumder, M. Ehsanul Haque; Haque, M. Ekramul; Islam, Anwarul
The present study was designed to investigate the antioxidant, anti-inflammatory and antipyretic potential of the methanolic extract along with its organic soluble fractions of the fruits of Trichosanthes dioica Roxb. Antioxidant potential of the extract/fractions was evaluated by DPPH (1,ldiphenyl-2-picrylhydrazyl) and NO (nitric oxide) scavenging assay method. Ethyl acetate fractions (EtOAc) showed highest scavenging activity in all the methods with IC50 value of 12.32±0.16 and 5.38±0.07 pg mL -1 for DPPH and NO assay method, respectively. In reducing power assay, EtOAc fraction also showed significant (p
In vitro and in vivo antioxidant and toxicity evaluation of different fractions of oxalis corniculata linn
(© 2011 Academeic Journals Inc., 2011) Alam, M. Badrul; Hossain, M. Sarowar; Chowdhury, Nargis Sultana; Mazumder, M. Ehsanul Haque; Haque, M. Ekramul; Islam, Anwarul
In course of investigation on natural antioxidants, the present study was aimed to report the antioxidant activities, both in vitro and in vivo, of the crude methanolic extracts of the whole plant of Oxalis corniculata Linn along with its various organic fractions. The different assay methods, including total antioxidant activity, scavenging free radical, authentic peroxynitrite, nitric oxide and reducing power assessment were used to evaluate the antioxidant potential of the crude extract and its organic fractions. The ethylacetate (EtOAc) fraction, showed strong activity in all the model systems tested and in peroxynitrite model this fraction (IC50 value of 2.29±0.18 μg mL-1) exerted three-fold stronger activity than standard penicillamine (IC50 value of 6.20±0.32 μg mL-1). The reducing power of the extract was found to be concentration dependent. The administration of the extract/fractions at a dose of 250 and 500 mg kg-1 body weight to the male Wistar rats increased the percentage inhibition of reduced glutathione, superoxide dismutase and catalase significantly. Whereas, lipid peroxidation level in hepatotoxic rats markedly decreased at a dose of 500 mg kg-1 body weight after 7 days. The total phenol and flavonoid content were also measured in the crude extract along with its organic fractions. The Brine shrimp lethality bioassay was used to determine the toxicity of the extracts and Vincristin sulphate was used as positive control. The dichloromethane (CH2Cl2) fraction showed highest activity (LC50 value of 29.02±1.16 μg mL-1) and other showed activity in the order of: EtOAc fraction >n-BuOH fraction > MeOH extract > aqueous fraction. Taken together, these results suggest that O. corniculata extract has strong antioxidant properties and further validate the traditional use of this plant.
In vitro antioxidant and anti-neoplastic activities of Ocimum sanctum leaves in Ehrlich Ascites Carcinoma bearing mice
(© 2011 Academic Journals Inc., 2011) Islam, M. Saiful; Alam, M. Badrul; Zahan, Ronok; Sarker, G.C.; Chowdhury, N.S.; Khan, M. Maruf; Hasan, Mahedi; Rahman, M. Saifur; Mosaddik, M.A.; Jesmin, Mele; Haque, M. Ekramul
The present study was designed to investigate the antioxidant and anti neoplastic activities of Ocimum sanctum Linn. (OS) leaves. Antioxidant potential of the OS extract was evaluated in vitro by DPPH (1, 1diphenyl-2-picrylhydrazyl) and NO (Nitric Oxide) scavenging assay and reducing power assay method. OS extract showed prominent scavenging activity in all the methods with IC50 value of 16.39±0.31 and 16.20±0.33 μg mL-1 for DPPH and NO assay method, respectively. In reducing power assay, OS extract also showed significant (p<0.001) activity. In addition, total phenolic and flavonoid content and total antioxidant capacity were also determined. The anti neoplastic effect of the leaves of OS against Ehrlich Ascites Carcinoma (EAC) in mice at the doses of 50 mg kg-1 body weight intraperit one ally. Significant (p<0.001) increases of survival times 33±1.81 days for crude extract of the (50 mg kg-1) treated tumor bearing mice were confirmed with respect to the control group (20±0.12 days). Hematological studies reveal that the Heamoglobin (Hb) content was decreased in EAC treated mice whereas restoration to near normal levels was observed in extract treated animals. There was a significant (p<0.001) decrease in RBC count and increase in WBC counts in extract/fraction treated animals when compared to EAC treated animals. From the result it was showed that the extract has significant antioxidant as well as anti neoplastic activity.
Phytochemical and Biological Investigation of the Plants Moringa Oleifera Lam. and Duranta Repens Linn
(University of Rajshahi, 2005) Nikkon, Farjana; Haque, M. Ekramul; Rahman, M. Habibur
The plant Moringa oleifera Lam. belongs to the family Moringaceae is locally known as "Sajna" (Eng. Drumstick) found to grow every., here in Bangladesh, India, Pakistan and other tropical and subtropical countries. It has various folk medicinal uses and effective against dysentery, diarrhea, cholera, pneumonia, scabies, relieves the pain of gout and acute rheumatism· etc. Isolation of two alkaloids, meringue and Moring nine and an antibacterial substance, pterygospermin have been reported. For chemical investigation, ethanolic extract of the dried powdered root barks of Moringa oleifera Lam. was successively fractionated with petroleum ether (40-60°C) and chloroform. The chloroform soluble fraction was subjected to a column of silica gel, eluted with n-hexane and ethyl acetate and their mixtures and re chromatography of the suitable portion afforded compound-I (31.4 mg) as needles, from petroleum ether, MP. 58-60°C. Compound-I was identified as deoxy-niazimicin (N-benzyl, S-ethyl thiocarbamate), a novel compound by analysis of its spectral data (IR, Mass and NMR) …………………….