Browsing by Author "Haque, Ekramul"

Now showing 1 - 10 of 10

Anti-alzheimer and antioxidant activity of celastrus paniculatus seed
(© 2011 Iranian Journal of Pharmaceutical Sciences, 2011) Alam, Badrul; Haque, Ekramul
The crude methanolic extract of the seeds ofCelastrus paniculatus along with its organic soluble fractions were tested for their possible antioxidant and antialzheirmer (AD) activity. The extracts showed prominent DPPH free radical scavenging activity, inhibiting activity of authentic peroxynitrite (ONOO-) and inhibition of total reactive oxygen species (ROS) generation. In DPPH radical scavenging assay, the EtOAc fraction showed the highest activity with a IC50 value of 25.92±1.02 mg/ml whereas aqueous fractions had no activity at all within the tested concentration. Scavenging of the authentic ONOO- system, all extract/fractions showed good activity and among them, EtOAc fraction had the highest activity with a IC50 value of 15.79±0.18 mg/ml. EtOAc fraction also showed significant (p<0.001) inhibitory activity against the total ROS generation which was almost similar with that of the positive control Trolox (IC50 16.79±0.19mg/ml). All extract/fractions exhibited statistically significant (p<0.001) cholinesterases (ChEs) inhibitory effects with IC50 values ranging between 134.7-227.5 mg/ml for AChE and 209.6-562.1 mg/ml for BChE.
Anti-alzheimer and antioxidant activity of celastrus paniculatus seed
(© 2011 Iranian Journal of Pharmaceutical Sciences, 2011) Alam, Badrul; Haque, Ekramul
The crude methanolic extract of the seeds ofCelastrus paniculatus along with its organic soluble fractions were tested for their possible antioxidant and antialzheirmer (AD) activity. The extracts showed prominent DPPH free radical scavenging activity, inhibiting activity of authentic peroxynitrite (ONOO-) and inhibition of total reactive oxygen species (ROS) generation. In DPPH radical scavenging assay, the EtOAc fraction showed the highest activity with a IC50 value of 25.92±1.02 mg/ml whereas aqueous fractions had no activity at all within the tested concentration. Scavenging of the authentic ONOO- system, all extract/fractions showed good activity and among them, EtOAc fraction had the highest activity with a IC50 value of 15.79±0.18 mg/ml. EtOAc fraction also showed significant (p<0.001) inhibitory activity against the total ROS generation which was almost similar with that of the positive control Trolox (IC50 16.79±0.19mg/ml). All extract/fractions exhibited statistically significant (p<0.001) cholinesterases (ChEs) inhibitory effects with IC50 values ranging between 134.7-227.5 mg/ml for AChE and 209.6-562.1 mg/ml for BChE.
Comparative study of antidiabetic activity of Cajanus cajan and Tamarindus indica in alloxan-induced diabetic mice with a reference to in vitro antioxidant activity
(© 2014 Pharmacognosy Network Worldwide) Nahar, Laizuman; Haque, Ekramul; Nasrin, Fatema; Mosaddik, Ashik; Zahan, Ronok; Haque, Anamul
Oxidative stress not only develops complications in diabetic (type 1 and type 2) but also contributes to beta cell destruction in type 2 diabetes in insulin resistance hyperglycemia. Glucose control plays an important role in the pro-oxidant/antioxidant balance. Some antidiabetic agents may by themselves have antioxidant properties independently of their role on glucose control. Objective: The present investigation draws a comparison of the protective antioxidant activity, total phenol content and the antihyperglycemic activity of the methanolic extract of Cajanus cajan root (MCC) and Tamarindus indica seeds (MTI). Materials and Methods: Antidiabetic potentials of the plant extracts were evaluated in alloxan-induced diabetic Swiss albino mice. The plant extracts at the doses of 200 and 400 mg/kg body weight was orally administered for glucose tolerance test during 1-hour study and hypoglycemic effect during 5-day study period in comparison with reference drug Metformin HCl (50 mg/kg). In vitro antioxidant potential of MCC and MTI was investigated by using 1, 1- diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity at 517 nm. Total phenolic content, total antioxidant capacity and reducing power activity was also assayed. Results: There was a significant decrease in fasting serum glucose level (P < 0.001), reduction in blood glucose level (P < 0.001) in 5-days study, observed in the alloxan-induced diabetic mice. The reduction efficacy of blood glucose level of both the extracts is proportional to their dose but MCC is more potent than MTI. Antioxidant study and quantification of phenolic compound of both the extracts revealed that they have high antioxidant capacity. Conclusion: These studies showed that MCC and MTI have both hypoglycemic and antioxidant potential but MCC is more potent than MTI. The present study suggests that both MCC and MTI could be used in managing oxidative stress.
Comparative study of antidiabetic activity of Cajanus cajan and Tamarindus indica in alloxan-induced diabetic mice with a reference to in vitro antioxidant activity
(© 2014 Pharmacognosy Network Worldwide) Nahar, Laizuman; Haque, Ekramul; Nasrin, Fatema; Mosaddik, Ashik; Zahan, Ronok; Haque, Anamul
Oxidative stress not only develops complications in diabetic (type 1 and type 2) but also contributes to beta cell destruction in type 2 diabetes in insulin resistance hyperglycemia. Glucose control plays an important role in the pro-oxidant/antioxidant balance. Some antidiabetic agents may by themselves have antioxidant properties independently of their role on glucose control. Objective: The present investigation draws a comparison of the protective antioxidant activity, total phenol content and the antihyperglycemic activity of the methanolic extract of Cajanus cajan root (MCC) and Tamarindus indica seeds (MTI). Materials and Methods: Antidiabetic potentials of the plant extracts were evaluated in alloxan-induced diabetic Swiss albino mice. The plant extracts at the doses of 200 and 400 mg/kg body weight was orally administered for glucose tolerance test during 1-hour study and hypoglycemic effect during 5-day study period in comparison with reference drug Metformin HCl (50 mg/kg). In vitro antioxidant potential of MCC and MTI was investigated by using 1, 1- diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity at 517 nm. Total phenolic content, total antioxidant capacity and reducing power activity was also assayed. Results: There was a significant decrease in fasting serum glucose level (P < 0.001), reduction in blood glucose level (P < 0.001) in 5-days study, observed in the alloxan-induced diabetic mice. The reduction efficacy of blood glucose level of both the extracts is proportional to their dose but MCC is more potent than MTI. Antioxidant study and quantification of phenolic compound of both the extracts revealed that they have high antioxidant capacity. Conclusion: These studies showed that MCC and MTI have both hypoglycemic and antioxidant potential but MCC is more potent than MTI. The present study suggests that both MCC and MTI could be used in managing oxidative stress.
Comparative study of antidiabetic activity of Cajanus cajan and Tamarindus indica in alloxan-induced diabetic mice with a reference to in vitro antioxidant activity
(© 2014 Pharmacognosy Network Worldwide) Nahar, Laizuman; Haque, Ekramul; Nasrin, Fatema; Mosaddik, Ashik; Zahan, Ronok; Haque, Anamul
Oxidative stress not only develops complications in diabetic (type 1 and type 2) but also contributes to beta cell destruction in type 2 diabetes in insulin resistance hyperglycemia. Glucose control plays an important role in the pro-oxidant/antioxidant balance. Some antidiabetic agents may by themselves have antioxidant properties independently of their role on glucose control. Objective: The present investigation draws a comparison of the protective antioxidant activity, total phenol content and the antihyperglycemic activity of the methanolic extract of Cajanus cajan root (MCC) and Tamarindus indica seeds (MTI). Materials and Methods: Antidiabetic potentials of the plant extracts were evaluated in alloxan-induced diabetic Swiss albino mice. The plant extracts at the doses of 200 and 400 mg/kg body weight was orally administered for glucose tolerance test during 1-hour study and hypoglycemic effect during 5-day study period in comparison with reference drug Metformin HCl (50 mg/kg). In vitro antioxidant potential of MCC and MTI was investigated by using 1, 1- diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity at 517 nm. Total phenolic content, total antioxidant capacity and reducing power activity was also assayed. Results: There was a significant decrease in fasting serum glucose level (P < 0.001), reduction in blood glucose level (P < 0.001) in 5-days study, observed in the alloxan-induced diabetic mice. The reduction efficacy of blood glucose level of both the extracts is proportional to their dose but MCC is more potent than MTI. Antioxidant study and quantification of phenolic compound of both the extracts revealed that they have high antioxidant capacity. Conclusion: These studies showed that MCC and MTI have both hypoglycemic and antioxidant potential but MCC is more potent than MTI. The present study suggests that both MCC and MTI could be used in managing oxidative stress.
Determinants of Hyperhomocysteinemia in Type 2 Diabetic Subjects
(University of Rajshahi, 2012) Akter, Kuhilika; Islam, Mohammad Amirul; Karim-2, Rezaul; Haque, Ekramul; Ali, Liaquat
Hyperhomocysteinemia is a recently recognized risk factor for cardiovascular disease that is independent of major risk factors such as diabetes, hypertension, hypercholesterolemia, and smoking. Although the mechanisms by which homocysteine promotes atherothrombosis are unknown, the epidemiological evidence for the ssociation of hyperhomocysteinemia with atherothrombotic disease is strong (Deland, Refsum, Brattstrom, 1992; Boushey et al, 1995; Welch, Loscalzo, 1998). Recent retrospective and prospective studies, it is now widely accepted that increased total plasma homocysteine is a risk factor for cardiovascular disease. Impaired enzyme function as a result of genetic mutation or deficiency of the essential B vitamins folic acid B 12 and B6 can lead to hyperhomocysteinemia. Several evidence suggests that it may play a role m atherothrombotic disease. Gradually increased homocysteine causes dysfunction of vascular endothelium. It was an observational study with a case-control design. Two Groups of subject were included in this study. I 08 subjects were diabetic without CVDs in Group I and IO 1 subjects were selected diabetic with CVDs in group II. A total of 209 subjects included in this study and its association with clinical, socio-economical and biochemical risk factors. Purposively and their economical, and clinical characteristics were noted in a predesigned case record form. Nutritional intake was assessed by food frequency questionnaire (24 hr dietary recall) method. Each subject went through OGTT following appropriate preparation and OM was diagnosed as per WHO Study Group Criteria. Blood glucose was measured by glucose oxidase method (Randox, UK) using a semi auto analyzer (300 MICROLAB). Lipids were measured by enzymatic methods using the same analyzer in the lab of Rajshahi Diabetic center. Plasma homocysteine concentration was measured by fluorescence Polarization Immunoassay (FPIA) technology (Abbott Laboratories, USA). Significance of differences will be analyzed by Student's t- test, as appropriate. Pearson's correlation and multivariate regression, as appropriate, were used to analyze the association between the other variables. Two Groups were age (yrs, M±SD) - matched and 55.4% male subjects were in Group II. BMI (kg/m2, M±SD); systolic blood pressure (mm of Hz) and diastolic blood pressure (mm of Hz) were significantly higher in Group II [(29±4.7); (130.5±12.9) and (88.4±8.6)] as compare to Group I [(24.1±3.8); (120.7±13) and (82.7±8)]; (p=0.013; p<0.001 and p=0.007) respectively. Fasting serum glucose level (mmol/1, M±SD) were significantly higher in Group II compared to Group I (18.5±6.01 vs 11.2±4.2; P<0.001). Serum glucose at 2 h after 75 g glucose administration were found to be (18.3±5.9) in Group I and (17.7±5.7) in Group II; (p=0.477). There was no significant difference in the two Groups. Sernm total cholesterol (mg/di, M±SD) were significantly higher in Group II compared to Group I (226±47 vs 207±34.2; P<0.001). Serum triglyceride (gm/di, M±SD) were significantly higher in Group II compared to Group I (218±85.5 vs 172±67.3; P<0.001). Serum HDL levels also significantly higher in Group I (47±8.1) as compare to Group II (39.8±10); (p=0.004). Serum homocysteine (µmol/L,, M±SD) were also significantly higher then compare to Group I (7.6±1.5) and Group II (14.5±5.8); (p<0.001). 37.6% subjects were in Group II their homocysteine level was below cut off value (<12 µmol/L) and 62.4% subjects were above cut off value of homocysteine level. BMI (kg/m2, M±SD); systolic blood pressure (mm of Hz) and diastolic blood pressure (mm of Hz) were significantly higher in Group II [(29±4.7); (130.5±12.9) and (88.4±8.6)] as compare to Group I [(24.1±3.8); (120.7±13) and (82.7±8)]; (p=0.013; p<0.001 and p=0.007) respectively. Fasting serum glucose level (mmol/1, M±SD) were significantly higher in Group II compared to Group I (18.5±6.01 vs 11.2±4.2; P<0.001). Serum glucose at 2 h after 75 g glucose administration were found to be (18.3±5.9) in Group I and (17.7±5.7) in Group II; (p=0.477). There was no significant difference in the two Groups. Sernm total cholesterol (mg/di, M±SD) were significantly higher in Group II compared to Group I (226±47 vs 207±34.2; P<0.001). Serum triglyceride (gm/di, M±SD) were significantly higher in Group II compared to Group I (218±85.5 vs 172±67.3; P<0.001). Serum HDL levels also significantly higher in Group I (47±8.1) as compare to Group II (39.8±10); (p=0.004). Serum homocysteine (µmol/L,, M±SD) were also significantly higher then compare to Group I (7.6±1.5) and Group II (14.5±5.8); (p<0.001). 37.6% subjects were in Group II their homocysteine level was below cut off value (<12 µmol/L) and 62.4% subjects were above cut off value of homocysteine level. The daily mean intake of dietary folate (mg/day, M±SD) in the study subjects as follows: as Group I (7.7±1.8) and Group II (1.8±0.93) respectively (p<0.001 ). Daily vitamin B6 as follows: as Group I (1.6±0.38) and Group II (1.0±0.61) respectively (p<0.001). And daily vitamin B12 as follows: as Group I (277±74.1) and Group II (119.1±29.8) respectively (p<0.001). There was significant difference in dietary intake folate, vitamins B6 and B12 in between the two Groups. On the coefficient correlation of homocysteine with other variables. From the data, a highly positive correlation of homocysteine was found with BMI, duration of OM, habit of exercise fasting blood glucose level and serum triglyceride (r=0.468, p= 0.050; t=0.392, p<0.001; r=0.181,p=0.009; r=0.332, p<0.001 and r=0.146; p=0.035) andnegative correlation was found between dietary folate, vitamin B6 and B 12 (r=- 0.563,p<0.001;1=-0.379 ,p<0.00 l and r=-0.484) respectively (p<0.001) among the Group II. On further analysis by multiple linear regressions Hyperhomocystenemia was found to have significant negative association only with dietary folate and vitamin B6 (􀀕=-0.408; p<0.001 and 􀀖=-0.128; p=0.053) when the effects of Age, BMI and groups were adjusted. From the above discussion it may be concluded that, Hyperhomocysteneimia is a important risk factor of cardiovascular disease in Bangladeshi diabetic population. Dietary deficiency of folate and vit B6 are the major determinants of hyperhomocystienemia in type 2 diabetic subjects.
Thank you, a good research antioxidant, antimicrobial and toxicity studies of the different fractions of fruits of Terminalia belerica Roxb
(© IDOSI Publications, 2011., 2011) Alam, Md. Badrul; Zahan, Ronok; Hasan, Mahedi; Maruf, M; Khan, Md. Maruf; S Rahman, Nargis; Chowdhury, M; Haque, Ekramul
The crude methanolic extract of the fruits of Terminalia belerica Roxb. along with its various organic fractions were tested to evaluate the toxicity, antioxidant (in vitro and in vivo) and antibacterial activity. The different assay methods, including total antioxidant activity, scavenging free radical, authentic peroxynitrite and reducing power assessment were used to evaluate the antioxidant potential of the crude extract and its organic fractions. While antimicrobial activities were evaluated using agar disc diffusion method and measuring their MIC value. The EtOAc fraction, showed strong activity in all the model systems tested and in peroxynitrite model this fraction (IC50= 1.29±0.18 μg/ml) exerted four-fold stronger activity than standard penicillamine (IC50 value of 5.20±0.32 μg/ml). The reducing power of the extract was found to be concentration dependent. 50 The administration of the extract/fractions at a dose of 200 and 400 mg/kg body weight to the male Wistar rats increased the percentage inhibition of reduced glutathione, superoxide dismutase and catalase significantly. Whereas, lipid peroxidation level in hepatotoxic rats markedly decreased at a dose of 400 mg/kg body weight after 7 days. EtOAc fractions showed also the highest antibacterial effect against Staphylococcus aureus with zone of inhibition 30.00±0.25 mm. The Brine shrimp lethality bioassay was used to determine the toxicity of the extracts where dichloromethane (CH2 Cl2) fraction showed highest activity (LC50= 39.02±1.16 μg/ml). In conclusion, the study clearly indicated that the extract/fractions of Terminalia belerica Roxb possesses good antioxidant and antimicrobial activity along with moderate toxicity.
Thank you, a good research antioxidant, antimicrobial and toxicity studies of the different fractions of fruits of Terminalia belerica Roxb
(© IDOSI Publications, 2011., 2011) Alam, Md. Badrul; Zahan, Ronok; Hasan, Mahedi; Maruf, M; Khan, Md. Maruf; S Rahman, Nargis; Chowdhury, M; Haque, Ekramul
The crude methanolic extract of the fruits of Terminalia belerica Roxb. along with its various organic fractions were tested to evaluate the toxicity, antioxidant (in vitro and in vivo) and antibacterial activity. The different assay methods, including total antioxidant activity, scavenging free radical, authentic peroxynitrite and reducing power assessment were used to evaluate the antioxidant potential of the crude extract and its organic fractions. While antimicrobial activities were evaluated using agar disc diffusion method and measuring their MIC value. The EtOAc fraction, showed strong activity in all the model systems tested and in peroxynitrite model this fraction (IC50= 1.29±0.18 μg/ml) exerted four-fold stronger activity than standard penicillamine (IC50 value of 5.20±0.32 μg/ml). The reducing power of the extract was found to be concentration dependent. 50 The administration of the extract/fractions at a dose of 200 and 400 mg/kg body weight to the male Wistar rats increased the percentage inhibition of reduced glutathione, superoxide dismutase and catalase significantly. Whereas, lipid peroxidation level in hepatotoxic rats markedly decreased at a dose of 400 mg/kg body weight after 7 days. EtOAc fractions showed also the highest antibacterial effect against Staphylococcus aureus with zone of inhibition 30.00±0.25 mm. The Brine shrimp lethality bioassay was used to determine the toxicity of the extracts where dichloromethane (CH2 Cl2) fraction showed highest activity (LC50= 39.02±1.16 μg/ml). In conclusion, the study clearly indicated that the extract/fractions of Terminalia belerica Roxb possesses good antioxidant and antimicrobial activity along with moderate toxicity.
Web Base Daily Online Shop
(Daffodil International University, 2021-11) Azad, Md. Mehedi Hasan; Haque, Ekramul; Milan, Md. Mehady Hasan
Our project is "a daily online shop" we can provide modern facilities in our customer because at this time people wants more facilities for shoping.so we want to give people this opportunity through this online platform .on our website the customer can easily create an account in our website and they can provide there review on the product and they can search many kinds of product very easily .customer chose any kind of product and they can add this product in cart and they will avail to order the product until it is out of stock .there are any problem in product after delivery customer can return product and company return money and this time customer no need to pay extra charge .any time they can chat with admin and asking about product and product details .We give best service and give best product .customer can pay in many ways cash on delivery and master card, visa card, bkash, nagad. There are three types of users admin, super admin, customer. Admin can add product ,cancel product ,delete information ,create a post ,cancel order .super admin add admin and delete admin.
Web Base Daily Online Shop
(Daffodil International University, 2021-11-05) Azad, Md. Mehedi Hasan; Haque, Ekramul; Milan, Md. Mehady Hasan
Our project is "a daily online shop" we can provide modern facilities in our customer because at this time people wants more facilities for shoping.so we want to give people this opportunity through this online platform .on our website the customer can easily create an account in our website and they can provide there review on the product and they can search many kinds of product very easily .customer chose any kind of product and they can add this product in cart and they will avail to order the product until it is out of stock .there are any problem in product after delivery customer can return product and company return money and this time customer no need to pay extra charge .any time they can chat with admin and asking about product and product details .We give best service and give best product .customer can pay in many ways cash on delivery and master card, visa card, bkash, nagad. There are three types of users admin, super admin, customer. Admin can add product ,cancel product ,delete information ,create a post ,cancel order .super admin add admin and delete admin.